Tiiu ban Khoa hgc cong nghe Biin • ISBN 978 604 913 015 1 BUOfC DAU LUtJ GILT MOT SO LOAI RONG BIEN \6l MUC DICH BAO TON PRELIMINARY STUDY ON STORAGE OF SEAWEEDS TO ESTABLISH EX SITU CONSERVATION Nguy[.]
Tiiu ban: Khoa hgc cong nghe Biin • ISBN: 978-604-913-015-1 BUOfC DAU LUtJ GILT MOT SO LOAI RONG BIEN \6l MUC DICH BAO TON PRELIMINARY STUDY ON STORAGE OF SEAWEEDS TO ESTABLISH EX-SITU CONSERVATION Nguyen Thi Huong, Le Nhu Hau, Vii Thi Mo" Vien Nghien ciiu va iTng dung Cdng nghe Nha Trang 02 - Hiing Vuong, Nha Trang, Khanh Hda Email: huong.nitra@.gmail.com Tom tat: Cd nhiiu bdng chirng cho thdy cdc lodi rong dang cd nguy ca tuyet chung vd thue su da cd mot sd lodi tuyet chimg vdng ndm gdn ddy Hiin nay, chua cd nhirng huang ddn cu thi nao cho viec tri cdc lodi cd nguy ca tuyet chimg ndy vd cd le cdc nhd khoa hoc nen xdy dung phirang phdp bdo tdn rong bien thdng qua nudi trdng nhirng ddi tuang ndy Xudt phdt tir thue ti dd, chiing tdi da nghien ciru Idm the ndo de luu giir rong biin phdng thi nghiim vai thai gian ddi nhdm bdo tdn cdc lodi rong quy hiim Ngudn Kappaphycus alvarezii (Doty), Gracilariopsis bailiniae (Zhang et Xia) va Gracilaria salicornia ((C Ag.) Dawson) dugc xir ly bdng Malachit Green (I2ppm) phiit Mdi trudmg nudi la nuac biin dugc bd sung MS (Murashige and Skoog Basal Salt Mixture) (0,05 mg/l) Rong biin da dugc luu trir tu dn dinh nhiet, vai nhiet nuac: 24 - 28 °C, man: 30-32 %o, pH; 7-8, chiiu sdng 12:12 (L: D) vai cuang 20-40^mol photon m' s', sue nhe Trong sudt qud trinh nudi, nuac biin vd chdt dinh duang dugc bd sung hai tudn I ldn Kit qud chi rdng rong bien cd the dugc luu tru Idu ddi (it nhdt, cho I ndm) phdng thi nghiem vai tdc tdng trudmg: 0,2-0,9%/ngdy Trong bdo cdo ndy, chung tdi trinh bdy kit qud ban ddu vi qud trinh luu giir mdt sd lodi rong biin diiu kien thi nghiim vai muc dich thdnh lap ngdn hdng gidng rong biin a Viet Nam tuong lai Abstract There is much evidence that algae are endangered and indeed that some of them have become extinct within recent years Currently, there are no guidelines for maintaining endangered species of algae Perhaps the scientific should establish guidelines for ex-situ conservation of algae in culture collections Thus, we have been studying how to maintain macroalgae in the laboratory for long time to preserving rare species of algae The source of Kappaphycus alvarezii (Doty), Gracilariopsis bailiniae (Zhang et Xia) and Gracilaria salicornia ((C Ag.) Dawson) were treated with Malachite Green (I-2ppm) for minutes The medium used for culture is seawater which was enriched MS (Murashige and Skoog Basal Sah Mixture) (0.05 mg/l) The seaweeds were stored in thermal stability cabinet, water temperature fluctuated between 24 and 28oC, salinity ranged from 30-32 %o, pH from 7-8, the photoperiod was maintained at 12:12 (L; D) and illuminated with 20-40pmol photon m-2s-I, light aeration conditions During this experiment, the culture 254 Hgi nghi Khoa hoc ky niem 35 ndm Vien Khoa hgc vd Cong nghe Viet Nam - Hd Ngi 10/2010 medium was replenished every two weeks The results indicate that these species can be stored for a long term (at least, for year) in the laboratory with growth rate; 0.2-0.9%/day In this report, we presented initial results on the culture collection in laboratory to establish gene seaweeds bank in Vietnam in the future Mddau Mdt sd loai rong cd gia trj kinh te da dugc nhieu nude tren the gidi nghien ciiu va dua vao nudi trdng de chii ddng ngudn nguyen lieu, nhien lieu cho cdng nghiep va thirc pham cho ngudi Ddng thdi, giai quyet cdng an viec lam, nang cao ddi sdng cho khdng it dan cu ven bien Tren thue te, ngudn gidng rong bien dua vao nudi trdng chii yeu la chgn loc nhiing cay khoe, sinh trudng tdt d vu trudc lam gidng cho vu sau ma chua cd ngan hang gidng thuan chiing de luu giir va chii ddng tao ngudn gidng can thiet Mot sd cdng trinh nghien cuu nhan gidng rong bang phuong phap nudi cay md cdng tren the gidi va nude da md nhieu trien vong cho muc dich luu giir va ling dung nudi trdng rong bien Mdt sd cdng trinh nghien ciiu cdng ciia cac nha khoa hoc tren the gidi ve nudi cay md, nhan gidng nhan tao, nudi trdng phdng thi nghiem tren ddi tugng Kappaphycus alvarezii bang mdi trudng ESS-1, PES da md nhieu trien vong cho muc dich luu giir va ling dung nudi trdng rong bien [2, 3, 4, 5] Trong dd d Viet Nam, mdi chi cd nghien ciiu thir nghiem cac phuong phap nhan gidng rong Kappaphycus alvarezii bang nhanh vdi viec sir dung dung djch khang sinh de khir triing va bd sung mdi trudng MS cho ket qua tuang ddi kha quan Tuy nhien, ket qua vdi muc dich nudi tang nhanh sinh khdi va chi nghien ciiu thdi gian ngan [1] Xuat phat tir thirc te va nhiing tun da dat dugc, chiing tdi di sau nghien ciiu lun giir mdt sd loai rong bien cd gia trj kinh te d dieu kien thi nghiem thdi gian dai, theo nhiem vu ciia de tai "Nghien ciru hiin trgng phdn bd vd gidi phdp bdo vi ngudn lgi rong Dd" cap Vien KH&CNVN (2009-2010), nham bao tdn dugc nhiing loai rong cd gia trj va chii ddng dugc ngudn gidng cho cac hoat ddng nghien cuu, nudi trdng khac Doi tuong va phuong phap nghien ciru 2.1 Doi tirang Ddi tugng dugc nghien cuu: Kappaphycus alvarezii (Doty) , Gracilariopsis bailinae{Zhang et Xia), Gracilaria salicornia {{C Ag.) Dawson) dugc thu tir viing bien Ninh Thuan va Nha Trang (thang - , nam 2009) Cac loai rong dugc dua ve phdng thi nghiem, thuan dudng be kinh trudc tien hanh luu giii Day la nhirng loai rong cd gia trj kinh te va da dugc nghien ciiu thdi gian gan day nen chiing tdi di sau nghien cim nham thiet lap quy trinh luu giii, budc dau hinh ngan hang gidng rong bien Viet Nam 2.2 Phirang phdp nghien ciru Chon nhiing nhanh rong khde, mau sac tuoi sang, khdng bj tray xudc Id loet Cat rong nhiing doan - 10 cm ddi vdi rong K alvarezii va giir nguyen nhanh rong ddi vdi Gracilariopsis bailinae, Gracilaria salicornia (10-12 g/nhanh) Rong dugc khu triing bang hon hgp khang sinh: Erymycine: 20 mg/l, Gentamycin: 12.8 mg/l Rifampin: 36 mg/l, Nergamycin: mg/l hoac Malachite Green (2ppm) Thdi gian khir triing: 2-5 phiit Sau khu triing, rira rong bing nude biin da loc qua ludi loc Phytoplanton de loai bd khang sinh hay hda chit cdn bam lai tren rong [1] 255 Tieu ban: Khoa hgc cong nghe Bien ISBN: 978-604-913-015-1 Mdi trudng dugc sir dung de luu giir: MS Nude biin dugc loc qua tiii loc Phytoplanton de loai bd thue vat ndi Hap nude bien ndi hip khir triing Thilt bj luu giii: binh nhua 600mL dugc dat tu In djnh nhiet Dilu kien lun giii: T°: 24-28°C, S%o: 3032%o, pH: 7-8, sue nhe, cudng chieu sang: 30-50|imol photon, 12 h/ngay Sau khir triing, rong dugc cho vao mot binh da cd sin 600mL nude bien va mdi trudng nudi Hang ngay, theo ddi su bien ddi ve mau sac, tinh trang rong va mdi trudng nude luu giii Thay nude, ve sinh binh va bd sung dinh dudng lian/2tuan Djnh ky kiem tra tdc tang trudng cua rong (llan/2 tuan), khir triing rong lan/thang Tien hanh luu giii rong d cac mdi trudng nude bien loc va khir triing vdi ndng 1/40MS (0.1 g/1) va 1/80MS (0.05g/l) dl chon loc mdi trudng tdi uu cac yeu td mdi trudng: Nhiet do: dieu chinh bang he thdng dieu nhiet tii dn djnh nhiet Do man: xac djnh bang khue xa ke Salinometer (Shibuya - Japan) Cudng anh sang: xac djnh bang may Li-2502 pH: bang may pH (Eco testr pHl) Khdi lugng rong dugc can bang can dien tir (KP, Maxel 500 - Taiwan) Rong dugc tham khd trudc can Xac djnh tdc tang trudng theo cdng thiic Penniman et al (1986) SGR IV 100 Trong dd: - SGR: tdc tang trudng tuong ddi (%/ngay) - WQ: lugng ban dau -1: thdi gian giira hai lan can (ngay) - Wt: lugng sau thdi gian t Ket qua va thao luan 3.1 Lu-u giii- lodi rong Sun (Kappaphycus alvarezii (Doty)) Nghien ciiu mdi trudng nude ya ndng dinh dudng thich hgp ddi vdi cac loai rong luu giir, chiing tdi nghien cim trudc hit ddi vdi loai K alvarezii Do ylu td khach quan, mot sd mau rong chit ddng loat sau 130 luu giir nen chiing tdi danh gia tdc tang trudng ciia loai d cac mdi trudng sau 126 Ngiy -Loc KhCr trving -Lpc + 1/40MS Lpc + 1/80MS -KhLP tmng •>• 1/40MS Khi> tmng + 1/80MS Hinh 2: Biiu thi hiin tdc tdng truang cua rong K alvarezii a cdc mdi truang Qua bieu (Hinh 2) thay dugc rong luu giir timg mdi trudng nude loc hay nude khir triing cd sir khac ve tie sinh trudng ddi vdi timg ndng dinh dudng bl sung Trong mdi trudng nude loc: rong dugc bl sung 1/80MS cd khdi lugng tang din theo 256 Hgi nghi Khoa hgc ky niem 35 ndm Vien Khoa hgc vd Cong nghe Viet Nam - Hd Ngi 10 2010 thdi gian lun giir nhung toe tang trudng tuong ddi lai thay ddi theo timg giai doan Tdc tang trudng tuong ddi ciia rong cd bd sung 1/80MS tang dan den thang thir rdi giam d cac thang tiep theo Tuy nhien, tic tang trudng d thang thii cd cao hon thang thir Trong dd, rong khdng bl sung dinh dudng hay bd sung 1/40MS, tdc tang trudng tuong ddi chi tang nhe d thang diu tien, thang thii giam dan va tang trudng am vao thang thii va thang thir Dac biet la vao thang thir 3, rong cd bd sung 1/40MS tdc tang trudng ciia rong la -5%/ngay Rong bj thdi nhiin va diit nhieu doan, mat mau iron bdng tu nhien Than rong bj teo quat lai ddi vdi nhirng mau luu giir mdi trudng khdng bd sung dinli dudng Ddi vdi mdi trudng nude khu triing: khdi lugng cac mau rong da sd tang len theo thdi gian luu giii ngoai trir mot sd mau rong dugc bd sung 1/40MS thi khdi lugng giam dan tir thang thii Tdc tang trudng tuong ddi ciia rong bd sung 1/40MS tang 42 dau sau dd giam dan, dac biet giai doan tir 84 tdi 98 tdc tang trudng la 1.46%/ngay Rong bj thdi nhiin d mot sd dinh nhanh va diit doan Trong dd rong khdng bd sung dinh dudng lai cd tdc tang trudng tang d 42 dau va nhiing thang thii Cac thdi gian cdn lai hau nhu khdng tang trudng, mau sac iron bdng tu nhien Bdng I; Tdc tdng truang tuang ddi ve khdi lugng cua rong Kappaphycus alvarezii Moi truong Toe tang trudng tuong doi ve khoi luffng (±SD) (%/ngay) Loc -0.6511'"'± 1.4959 Loc+ 1/40MS -0.9079' ± 1.9839 LOC+1/80MS 0.30517'" ±0.2896 Khir triing 0.1904"* ±0.2103 Khir triing + 1/40MS -0.2200'''±0.6011 Khir triing + 1/80MS 0.0003'" ± 2066 Ghi chii: sd trung binh cdt ling vdi cac chii sd (a, b, c, d) khac thi khac vdi (P