so what else is a dom good for anyway

... forming a cavity  11 A in height The size of this cavity is comparable with that of adeninelacking cobalamins, and thus allows the damaged 4940 Materials Crystalline AdoCbl was a gift from Eisai ... and a Grant in Aid for Natural Sciences Research (to T Toraya) from the Asahi Glass Foundation, Tokyo, Japan We thank Y Kurimoto for her assistance with manuscript preparation References Banerjee ... [25] ADP is bound to the ATPase domain, a core domain of the a subunit This domain shares common structural features with the ATPase domain of actin superfamily proteins, including Hsp70 molecular...

Ngày tải lên: 15/02/2014, 01:20

... Frisen J, Yates PA, McLaughlin T, Friedman GC, O’Leary DD & Barbacid M (1998) Ephrin -A5 (AL1 ⁄ RAGS) is essential for proper retinal axon guidance and topographic mapping in the mammalian visual ... of anti-placental alkaline phosphatase-agarose (anti-PLAP; Sigma) was packed into an FPLC column (Amersham Biosciences, Chalfont St Giles, UK) Purification of FN3d–AP was carried out using an AKTA ... cleavage sites (B) SDS ⁄ PAGE separation of FN3d–AP purified from conditioned media using anti-placental alkaline phosphatase (PLAP) agarose (C) SDS ⁄ PAGE and silver stain of proteins isolated...

Ngày tải lên: 19/02/2014, 05:20

... ATCCTTTATGGTGAGATCAGCTTCAATATCCGTCCA TTAAAGGCTATCAAATCGCAAGAACAGTTCACTAGA TCTAGTGAACTGTTCTTGCGATTTGATAGCCTTTAA GAAGCAAGAACAGTTCACTTAGTCAATTGATTAACGTG CACGTTAATCAATTGACTAAGTGAACTGTTCTTGCTTC Table Yeast ... Zymed (San Francisco, CA, USA) Horseradish peroxidaseconjugated goat anti-(mouse IgG) and gel-filtration standards were from Bio-Rad Laboratories (Hercules, CA, USA) Penta His mAb and Ni-NTA agarose ... other meiotic clade AAA ATPases and have the AAA domain helix and the C-terminal helix, but not the b domain The distinguishing feature of members of the meiotic clade of AAA ATPases is the SRH motif,...

Ngày tải lên: 07/03/2014, 05:20

... Glycan and glycosaminoglycan binding properties of stromal cell-derived factor (SDF) -1alpha Glycobiology 10, 21– 29 34 Valenzuela-Fernandez A, Palanche T, Amara A, Magerus A, Altmeyer R, Delaunay ... ligand for LESTR ⁄ fusin and prevents infection by T-cell-line-adapted HIV-1 Nature 382, 833–835 1950 N Charnaux et al Pablos JL, Amara A, Bouloc A, Santiago B, Caruz A, Galindo M, Delaunay T, ... immunofluorescence and FACS analysis SD-4 mRNA, SD-1 mRNA, SD-2 mRNA and CXCR4 mRNA and, to normalize for input of total RNA, glyceraldehyde 3-phosphodehydrogenase (GAPDH) mRNA were quantified by RT-PCR Total...

Ngày tải lên: 16/03/2014, 18:20

... 5¢-GTGGAGGACATCTATGGGCAGCAG-3 Gly896fiArg 5¢-GTGGAGGACAGCTATAGGCAGCAG-3¢ Gly896fiIle 5¢-GTGGAGGACAGCTATATCCAGCAG-3¢ a Second primer for all of the above 5¢-GTCATTAATCCAACGGTCATCCCA-3¢ Primers used for ... 5¢-GTGGAGGACAGCTATGGGCAGCAG-3¢ Wild type Asp893fiArg 5¢-GTGGAGCGCAGCTATGGGCAGCAG-3¢ Asp893fiGlu 5¢-GTGGAGGAGAGCTATGGGCAGCAG-3¢ Asp893fiAla 5¢-GTGGAGGCCAGCTATGGGCAGCAG-3¢ Ser894fiAsp 5¢-GTGGAGGACGACTATGGGCAGCAG-3¢ Ser894fiIle ... properties, although in somewhat different ways In general, however, we can distinguish between catalytically inactive and catalytically active mutants Catalytically inactive mutants All mutants of Asp893...

Ngày tải lên: 23/03/2014, 13:20

... or His137, residues acting as the general acid and base on the catalysis by a- sarcin [19,20], rendered proteins with no detectable activity against ApA [20] Cleavage of ApA by a- sarcin is a low-specificity ... proposed that the mechanism of action of a- sarcin against ribosomes might differ in some details from that against dinucleotides [18,19] The behaviour of a- sarcin against ApA as a function of pH, altogether ... plays an important role in the rRNA catalytic cleavage by a- sarcin, which would not be simply related to the bearing positive charge Both mutant variants of a- sarcin cleave ApA, with slight variation...

Ngày tải lên: 31/03/2014, 23:20

... DNA-shift assay PSEx1 PEx1R simA7F CCATGTAGGCGGTGACGA TAAAGCTTCAAAACGGGGTGAAC DNA-shift assay PA7 simA7R ATAAGCTTGTCGATACCGATCTTC DNA-shift assay PEx2 DNA-shift assay PSR3 PEx2F ACTTCCCAGAAGTA ... TAGAATTCATCGCCACGACCATG SD2R1R TAGAATTCCGCGGTTCGGCAGA simX5D3F TAGAATTCTGTACAAGGCCTGGT DNA-shift assay PD3 simX5D3R simEXX4F TAGAATTCGCGACAGGAGCCATA TAGAATTCGACGCCTTCCAGTC DNA-shift assay PX4 simEXX4R TAGAATTCTCAGAACATCGTCC ... ATACCATGGCCCGTGAACGT SimReg1 simReg1 SSR1R TTTGAATTCATTAATGGTGATGGT purification DNA-shift assay PD4 DNA-shift assay PR1 SR1D4F TAGAATTCGTGAGCAGATCATGT SR1D4R TAGAATTCCATTGTGAACCATC SD2R1F TAGAATTCATCGCCACGACCATG...

Ngày tải lên: 21/06/2014, 17:20

... mutual love and support These lessons are much more practical than the abstract and theoretical ones taught in school Therefore the above sentence offers us a valuable lesson about the importance ... in a country, which always pays close attention to the role of the family and regards family as a basic unit of society But in the society where family is not considered as the foundation of society, ... must have a general knowledge in order that she can satisfy her child’s thirst for knowledge From this point of view, family is the very base of the school education Family is a good school Family...

Ngày tải lên: 22/07/2014, 04:20

... Tsuchiya KJ, Takagai S, Kawai M, Matsumoto H, Nakamura K, Minabe Y, Mori N, Takei N: Advanced paternal age associated with an elevated risk for schizophrenia in offspring in a Japanese population Schizophr ... order/rank and schizophrenia may be due to paternal age In fact, as advanced paternal age was a risk factor for schizophrenia, the disease frequency was greater in children with higher birth rank as ... rare human disease has prompted research on complex common disease to focus on genetic approaches Table Sample classes as a function of parental or maternal age at birth Group Parental age at...

Ngày tải lên: 09/08/2014, 01:21

... TCTCCCTCCAAGCAGGTCGAAGAAGGGGGCAAAGCAGACACCCTGAGCTCC TCTCCCTCCAAGCAGGTCGAAGAAGGGGGCAAAGCAGACACCCTGAGCTCC TCTCCCTCCAAGCAGGTTGAAGAAGGGGGCAAAGCAGACACCCTGAGCTCC TCTCCCTCCGAGCAGGTTGAAGAAGGGGGCAAAGCAGACACCCTGAGCTCC t p.G60V ... Gorilla gorilla Pongo pygmaeus Macaca mulatta Callithrix jacchus TCTCCCTCCAAGCAGGTCGAAGAAGGGGGCAAAGCAGACACCCTGAGCTCC TCTCCCTCCAAGCAGGTCGAAGAAGGGGGCAAAGCAGACACCCTGAGCTCC TCTCCCTCCAAGCAGGTCGAAGAAGGGGGCAAAGCAGACACCCTGAGCTCC ... manuscript SL and TS evaluated and interpreted SNP microarray and aCGH data All authors read and approved the final manuscript for publication Acknowledgments We thank the heterotaxy patients and families...

Ngày tải lên: 09/08/2014, 23:20

... plasma viral load, age, education, adherence, and history of IDU were associated with HIV plasma viral load response The univariate and multivariate associations of aboriginal status, clinical and ... information on socio-demographic characteristics, there is always a chance for missing information To date several studies dealt with missing information via assumptions about the missing data or ... baseline HIV plasma viral load, age, physician experience and adherence Table shows the results for the univariate and multivariate associations of Aboriginal status, clinical and sociodemographic...

Ngày tải lên: 10/08/2014, 05:20

... there are databases for standard NMR and mass spectra, including the Human Metabolome Database [13] and the Madison Metabolomics Consortium Database [14] There are also databases that are already ... database must capture all this information but clearly this is not feasible However, these problems face any data standard and this will not just affect metabolomics, but also be a problem for ... but what you can for data standards! Abbreviations EBI, European Bioinformatics Institute; HUPO, Human Proteome Organization; MGED, Microarray Gene Expression Database; MIAME, Minimum Information...

Ngày tải lên: 11/08/2014, 12:20

... nuclear matrix proteins Proc Natl Acad Sci USA 1991, 88:10312-10316 51 Hisada-Ishii S, Ebihara M, Kobayashi N, Kitagawa Y: Bipartite nuclear localization signal of matrin is essential for vertebrate ... 5′GTCTCTCTGGTTAGACCAG-3′, Primer C 5′-CTAGTCAAAATTTTTGGCGTACTC-3′ and primer A and sj4. 7A 5′- TTGGGAGGTGGGTTGCTTTGATAGAG-3 for spliced Kb transcript For GAPDH forward 5′ CTCTGCTCCTCCTGTTCGAC 3′ and GAPDH ... http://www.retrovirology.com/content/8/1/61 reading and commenting on the manuscript, and Barbara Felber for sharing several critical reagents We are grateful to Anna Kula and Alessandro Marcello for sharing data in their paper prior...

Ngày tải lên: 13/08/2014, 01:21

... supported an asthma diagnosis Most patients with asthma are not cared for by asthma specialists and frequently the diagnosis is made on clinical grounds without formal pulmonary function testing Additionally, ... infiltrates or arterial hypoxemia The study excluded children with an exacerbation of reactive airways disease If patients with sickle cell disease are at increased risk of airway inflammation and ... both asthma and sickle cell disease had increased hospital admissions for pain crises and acute chest episodes compared with patients with only sickle cell disease [9] Our results are unique because...

Ngày tải lên: 13/08/2014, 13:22

... with FACS Lyse (BD Catalog Nr 349202) Data was acquired for minutes from all patients at all visits Each day data was acquired, compensation controls were acquired, and data was compensated before ... basophil activation as measured by upregulation of CD63 and CD203c was investigated by a Bland Altman analysis [19] Blood basophil concentrations determined by constant volume acquisition (CVA) ... the allergen concentration at which half-maximal activation (LC50) is achieved (e) LC50 was normalised to baseline and plotted against visit number Mikkelsen et al Clinical and Molecular Allergy...

Ngày tải lên: 13/08/2014, 13:22

... endothelial barrier [53] In addition, HMGB1 acts as a pro-inflammatory factor, and is released by damaged cells as a chemoattractant for vascular smooth muscle cells and fibroblasts and induces ... Palumbo et al reported that HMGB1 attract mesoangioblast to migrate across an endothelial monolayer and reach damaged muscle HMGB1 therefore appears to induce stem cell transmigration across an endothelial ... A major obstacle of utilizing NSCs in clinical applications is the need for fetal brain tissue There is limited availability of fetal tissue and it raises multiple moral and ethical complications...

Ngày tải lên: 09/09/2015, 08:17

... Phosphate buffer saline Phosphatidylcholine Parkinson’s Disease PTEN-induced putative kinase Polyunsaturated fatty acids Standard error of mean Substantia Nigras par compacta Substantia nigras par ... ester linked fatty acyls (B) and plasmalogens (C) are found in high levels in the brain Ethanolamine plasmalogen has an ether linkage A major fatty acyl brain PE is arachidonic acid (AA, 20:4), with ... age-related neurodegenerative disorder (Elbaz and Moisan, 2008) The clinical characterization of the disease, started about half a century ago, is extremely accurate Among experienced clinicians,...

Ngày tải lên: 10/09/2015, 15:52

... developmental studies in vertebrate Zebrafish has many advantages over other vertebrate organisms Firstly, rapid external development makes translucent embryos available for visual analysis of tissue formation ... morphogenesis in zebrafish 1.3.4.1 Exocrine and endocrine pancreas in zebrafish Like the mammalian pancreas, zebrafish pancreas is also composed of two tissues, endocrine pancreas and exocrine pancreas, ... serves as a tissue for haematopoiesis during gestation, the vascular development in liver is essential Angioblasts, precursors of endothelial cells, start to appear near the hepatoblasts at the stage...

Ngày tải lên: 11/09/2015, 16:06

... must lead, motivate, inspire, and encourage them… • Good managerial skills are all about planning and utilising time, resources and man power to get the work done A leader is a visionary, who ... they can learn and develop further 5)wise in the handling of document • A good manager must know how in the processing data Maybe managers are afraid of cumbersome paperwork but the manager also ... ideas can flow and flourish, follow some of these basic leadership and management practices 7)Information processing and thinking capacity • The power of your brain is incalculably large The manager...

Ngày tải lên: 26/12/2014, 08:36