nad p h in isolated arteries during hypoxia

... Đình Cường, Lê Thị Thu Hiền, Nơng Văn Hải, Đặng Thành Nam, Phan Văn Chi (2004), "Tinh chế phân tích khối phổ protein bất hoạt ribosome tái tổ hợp từ mướp đắng (Momordica charantia L.) ", Tạp chí ... đến hoạt động enzyme NQO1 bám vào giúp ổn định nhân tố ức chế khối u p53 chống lại phân hủy proteasomal Hơn NQO1 xuất để điều khiển tính phân hủy protein khác Những phát cho thấy vai trò chọn ... Phân lập thiết kế vector mang gen mã hóa NAD(P)H quinone oxidoreductase 1(NQO1) người Nguyễn Thị Thanh Nga Trường Đại học Khoa học Tự nhiên Luận văn ThS Chuyên ngành: Sinh học thực nghiệm;

Ngày tải lên: 15/12/2017, 04:27

... Protein tổng số mẫu tế bào HEK mang plasmid tái tổ hợp pcDNA3.1B-NQO1 57 Nguyễn Th Thanh Nga K20 Sinh học thực nghiệm Protein tái tổ hợp NQO1 có gắn 6x-histidine nên protein tái tổ hợp tinh hệ thống ... Đình Cường, Lê Thị Thu Hiền, Nông Văn Hải, Đặng Thành Nam, Phan Văn Chi (2004), "Tinh chế phân tích khối phổ protein bất hoạt ribosome tái tổ hợp từ mướp đắng (Momordica charantia L.) ", Tạp chí ... bào HEK293 Kiến ngh  Tiếp tục nghiên cứu biểu cDNA mã hóa NQO1 vector pcDNA3.1B tế bào HEK293  Nghiên cứu phương pháp thu hồi tinh protein NQO1 59 Nguyễn Th Thanh Nga K20 Sinh học thực nghiệm

Ngày tải lên: 15/09/2020, 14:48

... mM Tris/Cl, pH 54.5 52.5 50 mM HEPES, pH 56.0 54.0 Holoprotein in 50 mM HEPES, pH +SYPRO® Orange 52.8 51.2 Apoprotein in50 mM HEPES, pH +SYPRO® Orange 50.8 48.5 Table 2: Reductive half reaction ... Importantly, SAXS measurements of wild-type NQO1 showed that the protein forms a dimer in solution both in the holo- and the apo-form, with the holo-protein being more compact compared to the ... pockets in the homodimeric protein (Figure 3; data with wild-type) Interestingly, we observed that variable amounts of protein had apparently precipitated during the experiment Thus, a possible

Ngày tải lên: 19/11/2022, 11:48

... cells, indicating that NSDHL might be largely involved in cholesterol synthesis pathway in MDA-MB-231 cells We found that a significant increase in S phase and cell cycle arrest in G0/G1 phase in ... group expressed less NSDHL mRNA and protein than the MCF10A cell line We observed high level of NSDHL protein expression in basal-like and TN subtype (BT-20 and MDA-MB-231) compared to the other ... = 5) (Fig 5d) The representative IHC for NSDHL showed strong staining in the control tumor and overall weak staining in the NSDHL-knockdown tumor The NSDHL-stained cells in NSDHL-knockdown tumor

Ngày tải lên: 30/05/2020, 22:04

... genotypes of the avian influenza viruses during their evolution The Southern China has been shown to be the avian influenza virus pool for flu outbreaks in history, including H2N2 (1957), H3N2 ... genotype of H5N1 AIV identified in southern China 1263 isolates, so called genotype Z [12], especially those that isolated from duck in Hunan Province, China [12] (Fig 1A) However, the internal ... southern China 1261 Figs 1A, B Phylogenetic analyses of the H5N1 AIVs isolated from Southern China during 2003–04 H5N1 outbreaks A The HA genes of all isolates have a close linkage to other 2003–04

Ngày tải lên: 18/10/2022, 17:52

... August, especially women Table 5 shows that income per capita per month of Kinh people is highest with 332 thousand VND, while Chau Ma people has on the average of 236 thousand VND and Stieng people ... groups in village 4, the finding shows that there are differences in their lives This part will go deeper into the detailed characteristics of the people Household survey provides the data in this ... Nearly half of Chau Ma and Stieng groups have five to six persons per household Meanwhile, over half of the Kinh group have three to four persons per household Therefore, household size of Chau

Ngày tải lên: 02/03/2024, 14:21

... TTP, 6%. Poly(P)s (pyrophos- phate, tripolyphosphate and trimetaphosphate at 5 mm and polyphosphate, metaphosphate and hexametaphos- phate at 1 mgÆmL )1 ) and phosphorylated compounds (phosphocreatine, ... accepted 3 May 2005) doi:10.1111/j.1742-4658.2005.04749.x ATP-NAD kinase phosphorylates NAD to produce NADP by using ATP, whereas ATP-NADH kinase phosphorylates both NAD and NADH. Three NAD kinase ... 3337 phosphoryl donor, the enzyme using ATP and poly(P) is termed poly(P) ⁄ ATP-NAD kinase [4] and that using ATP, but not poly(P), is termed ATP-NAD kinase [6]. For the phosphoryl acceptor, the

Ngày tải lên: 20/02/2014, 01:20

... at the juncture when they appeared, and in the numbers in which they came, greatly contributed to encourage their brethren who were then passing through the heat and fire of the conflict, as well ... immediately apparent. In the early part of September, war meetings were being held in Pennsylvania to raise the quota of the State in lieu of the draft then impending, in pursuance of the requisition ... Mayor McKnight presided over the meeting. Dispatches were read indicating the approach of the enemy to the borders, and resolutions were adopted to organize companies forthwith in each of the wards.

Ngày tải lên: 21/02/2014, 21:20

... with chromatin. In some of these proteins, such as heterochromatin protein 1 (HP1), the chromodomain has been shown to specifically interact with histone tails bearing methylated lysines [7]. In ... spermatogenesis. Site-specific phosphorylations of H2A [71], H2AX [40] and H2B [72] have also been reported. While nothing is known a bout the phosphorylation of H2A and H2B during spermatogenesis, that of H2AX ... modifications has recently led to the proposal of the Ôbinary switchesÕ hypothesis modulating the readout of specific marks such as lysine methylation [91]. In fact the phophorylation of Thr/Ser in Thr/Ser-Lys

Ngày tải lên: 07/03/2014, 16:20

... of Pi [Pi]ot P2o ẳ Pt o Ho ỵ K P;H Ho P1o ẳ P t K P;H ỵ KHo P;H ð8Þ The Pi ⁄ H carrier of mitochondria catalyzes the electroneutral symport of twice-protonated phosphate and proton: H2PO4) + Ho+ ... protons to ⁄ from Pi to form the complexes H2PO4) and HPO42) in the mitochondrial matrix and outside of mitochondria HPO2 ỵHỵ ẳ H2 PO ; K P;H ¼ 4 P2i ÁHi P2o ÁHo ; K P;H ẳ P1i P1o 1ỵ Mgi KT;Mg ... Mitochondria with nonfunctional respiratory chains become ATP consumers, maintaining an appreciable PMF by pumping protons out of the matrix through the F0 ⁄ F1-ATPase, at the expense of ATP hydrolysis

Ngày tải lên: 16/03/2014, 00:20

... of the valley, where they buriedherinaditch.Thatnight,whenthemiserheardofher death, hedroppeddowndeadonthespot.  Astheladwasgoinghomethequeenappearedtohim,andtoldhim tobeattheringthefollowingdayatnoon.  WelshFairyTales 6 VI.  Nextdayallthefairiescamewiththepoleandmirror,eachcarrying aharebellinherleft‐hand,andabluecupofburningperfumeinher right, ... built  in  some masonry.    When he had tied the horse up he went to shoe the off hind‐leg, but  directly he touched the horse the spirited animal started back with a ... they  that the tribes had to protect their village with high walls, [Footnote:  ... the hill on which he stood she gained on them.Asshecametowardsthemillhethrewhisstone,anditlodged in her skull, and when he ran up he found he had killed the 

Ngày tải lên: 16/03/2014, 15:20

... shows sections from the overlaid 2D 1 H– 15 N HSQC spectra of the H1–47 domain (shown in blue) and the H1–47–E1–100 complex (shown in red), highlighting differences in terms of changes in chemical shift ... sodium phosphate buffer, pH 6.5, for 10 min at room temperature. The excess non- bound dye was removed five times using a ZipTipÒ P-10 pipette tip with C4 resin (Millipore), replacing the solution with ... mainly interacts with subunits H and D via its N- and C-ter- minal parts, respectively. In summary, the data presented support the view that the a-helical subunit H forms one of the two peripheral

Ngày tải lên: 30/03/2014, 04:20

... on an intermedi- ate pH value, i.e. 4.75, which is the optimum pH for trehalose assimilation [14]. Although suboptimal, the growth on trehalose remains possible in a broader range of pH (4.8 ... oscillatory phenomenon, which is the sum of two sine functions [y ¼ sin (3x ⁄ 2) + sin x] the theoretical periods of which are P 1 ¼ 3p ⁄ 2 (4.189 h) and P 2 ¼ 2p (6.283 h), respectively (vertical lines, ... fermentative activity. The second type of oscillation, which was also observed in both wild-type and agt1 mutant strains, had a shorter period that was independent of the specific growth rate. Accordingly, the period

Ngày tải lên: 30/03/2014, 16:20

... out the possibility that the lack of catch effects during FF 50% exposure were due to a large mechanical impedance around the ankle during push-off, and supports the interpretation that the subjects ... experiments are required to address this point. Competing interests The authors declare that they have no competing interests. Authors' contributions MN participated in the design of the ... ankle plantarflexors provide power to propel the center of mass of the body forward. Increase in power gen- eration during this phase leads to increases in gait speed. Push-off deficits have been

Ngày tải lên: 19/06/2014, 08:20

... 2Hb and CO2Hb concentration changes during BH Average O Average O2Hb and CO2Hb concentration changes during BH O2Hb (left graph) and CO2Hb (right graph) concentrations during BH for the sample ... one year, the subject improved his motor and phasic performances The results of the BH test reveal the effects of the therapy: the BHIV increases and the PI shows a drop during BH, meaning a little ... diagrams of the BHIV and of the slopes (O2Hb on the left panel and BHIO2 CO2Hb on the right panel) for our sample population The black line represents the linear regression of the data The Pearson's

Ngày tải lên: 19/06/2014, 10:20

... lower than that in the falls risk elderly. Higher mean MFC in the falls risk elderly group supports our previous findings [4] which showed that increasing the MFC height is one of the possible strat- ... ApEn of MFC The algorithm for estimating ApEn of heart rate was first reported by Pincus [8]. We explain that approach as applied to MFC data. ApEn is defined as the logarithmic likelihood that ... absent in the falls-risk group. The ApEn values in the falls-risk group (mean ApEn = 0.18 ± 0.03) was significantly (p < 0.05) higher than that in the healthy group (mean ApEn = 0.13 ± 0.13). The

Ngày tải lên: 19/06/2014, 10:20

... the initiation and propagation of hypoxia/ischemia-induced inflammation by secreting inflammatory chemokines that attract neutrophils and monocytes into the brain However, triggers of chemokine ... ARC-814, Philadelphia, PA 19104, USA and 4Visiting Scholar from the Beijing Friendship Hospital affiliated to the Capital University of Medical Sciences, Beijing, China Email: Jelena Mojsilovic-Petrovic ... Neuroinflammation BioMed Central Open Access Research Hypoxia-inducible factor-1 (HIF-1) is involved in the regulation of hypoxia-stimulated expression of monocyte chemoattractant protein-1 (MCP-1/CCL2)

Ngày tải lên: 19/06/2014, 22:20

... also increased during the autumn, when air temperature higher magnitudes than during the previous warm period, despite the much lower decreased vapour pressure deficit at that time The progression ... uptake for explain the decreased pine during the spring period (figure 4) In spring of 1995, water was abundant at the site and also spruce transpiration matched the predicted rates of E at that ... reduction in gross photosynthesis for pine and spruce, respectively, indicating a loss of production of at least that proportion Pines were less sensitive to drought spells as compared to the more shallow-rooted

Ngày tải lên: 09/08/2014, 04:20

... constitute the first report that CaR plays an important role in the SR-mitochondrial inter-organelle Ca 2+ signaling through the IP 3 Rs, which are also involved in apoptosis during H/Re. Additional ... involved in the release of Ca 2+ from the SR through the IP 3 R during H/Re. Rizzuto et al. have provided a structural basis for this hypothesis by showing that mitochondria and ER form an interconnected ... cytoplasm to the mitochondria and the release of cytochrome c from the mitochondria dur- ing H/Re. The membrane receptor CaR couples to the enzyme PLC, which liberates IP 3 from phosphatidylinositol

Ngày tải lên: 10/08/2014, 05:21

... assets is apparent when we compare the balance sheet of U.S. households, shown in Table 1.1, with the composition of national wealth in the United States, shown in Table 1.2. Household wealth includes ... shareholders in principle can elect a different board. They can do this by launching a proxy contest in which they seek to obtain enough proxies (i.e., rights to vote the shares of other shareholders) ... wealth among investors. Individuals can choose between consuming their wealth today or investing for the future. If they choose to invest, they may place their wealth in financial assets by purchasing

Ngày tải lên: 10/08/2014, 07:21