molecular cross talk between nuclear receptors and nuclear factor kb

... complex between nuclear factors and the double stranded PNA –DNA–PNA chimera mimicking the HIV-1 LTR NF -kB binding sites, in order to investigate the molecular interactions between nuclear factors and ... NF -kB PNA–DNA chimera [30] and prediction of molecular interactions between the PNA–DNA chimera and nuclear proteins belonging to the NF -kB family was performed by energy minimizations and molecular ... prediction of the molecular interactions between a double-stranded PNA – DNA –PNA chimera and nuclear proteins belonging to the NF -kB family was performed by energy minimization and molecular dynamics...

Ngày tải lên: 24/03/2014, 04:21

... Reciprocal activation between NK and THP-1 cells, the role of IL-15 and IFN-γ (a) IL-15 and β2 integrins are involved in the intercellular contact with and THP-1 cells, the role of IL-15 and IFN-γ THP-1 ... mAb) and CD48 (the CD244 ligand) increased TNF synthesis (Figure 4b) It is noteworthy that NK cells and monocytes express both CD48 and CD244, whereas THP-1 cells express only CD244 (Figure 5a and ... the study, performed statistical analysis and drafted the manuscript CD-J and VN-G purified cells and performed co-culture assays of both PBL and SFL and performed enzyme immunoassays AMO performed...

Ngày tải lên: 09/08/2014, 08:22

... flexible linker between the AC subdomain and the PCP domain, and the absence of a contact surface between these two folded units [19], argue for a certain degree of flexibility and mobility of ... solvent Taken together, our results support significant conformational changes in the crosstalk between A domains and PCP domains that reflect different states of the PCP domain in the catalytic cycle ... secondary structure, between the A domain and the PCP, which probably allows the PCP to travel between different catalytic centres In fact, this linker constituted the only connection between the two...

Ngày tải lên: 29/03/2014, 08:20

... article as: Liu and Huber: Cross- talk between cd1d-restricted nkt cells and gδ cells in t regulatory cell response Virology Journal 2011 8:32 Submit your next manuscript to BioMed Central and take full ... antigen-specific CD4+ and CD8+ T cells, and promoting inducible Tregulatory cell activation through release of TGFb, IL-10 and IL-2 TNFa and IFNg secretion by Vg4+ T cells enhances maturation and antigen ... T cell receptors (TCR) and NK receptors There are two types of NKT cells Type NKT cells have a TCR comprised of a single type of TCRa chain (Va14Ja18 for mice and Va24Ja18 for humans) and one...

Ngày tải lên: 11/08/2014, 21:21

... estrogeninduced responses in the rodent uterus, mammary gland and in the mammalian cell cultures (reviewed in [12,13]) The cross- talk between the ERα and AHR signalling pathway has not been extensively ... [41,42] It has been shown that upon ligand binding and nuclear translocation the AHR heterodimerize with nuclear ARNT, and then associates with the unliganded ERα or ERβ The resulting complex ... interference with the liganded estrogen receptor cross- talk between aryl hydrocarbon- and estrogen-mediated signaling Journal of Biological Chemistry 1996, 271:10533-10537 Nodland KI, Wormke M, Safe...

Ngày tải lên: 13/08/2014, 13:20

... Charbonnier AS, Winter D, Amatschek S, Tamandl D, Schweifer N, Stingl G, Maurer D Balance between NF-κB and JNK/AP-1 activity controls dendritic cell life and death Blood 2005, 106, 175-183 12 Kubo ... taxol for 24 or 48 h The supernatants were harvested from the cultures and used for the determination of IL-12 and tumor necrosis factor- α (TNF-α) production, both of which are important cytokines ... paraformaldehyde-containing phosphate-buffered saline and then stained with μg/ml tetramethylrhodamine isothiocyanate-labeled anti -nuclear factor- κB (NF-κB) p65 monoclonal antibody (Santa Cruz...

Ngày tải lên: 07/08/2014, 23:22

... include growth factors, cytokines and enzymes and has been linked with hormone-independent growth, loss of cell-cell adhesion and angiogenesis Proliferation and apoptosis The mitogenic and growth-promoting ... adenocarcinoma [41] Nuclear and cytosolic AR expression in two newly established human oesophageal carcinoma cell lines (ES-25C and ES-8C) has also been shown [42] Androgen receptors may be important ... ligand to oestrogen response elements involving instead protein-protein interactions The most prominent for ERa appear to be the transcription factors, specificity protein (SP-1) and nuclear factor...

Ngày tải lên: 09/08/2014, 03:21

... significant links that we recovered between Ynl155w and the splicing factor Snu13 are also reminiscent of the earlier detected link between the splicing factor Brr2 and the ERAD system protein Sec63 ... components in yeast and then assemble all their known physical, genetic and biochemical interactions between themselves and with the rest of the proteome Graphs or networks have become the standard representation ... respect to sumoylation and ubiquitination Sumoylated proteins are clearly overrepresented in the nuclear compartment (including nucleoplasm, nuclear pore, nucleolus and nuclear periphery; FET,...

Ngày tải lên: 14/08/2014, 21:20

... grateful for the advices and suggestions given by Dr Cui Z Z (Animal Science and Technology College, Shandong Agricultural University, China) and for the critical review and suggestions of Dr Xingquan ... rMd5-infected CEF cells transfected with plasmids pPGA(pp38)-CAT and pPGA(1.8 kb) -CAT Transfected cells were harvested and lysed by repeats of freeze and thraw The lysed samples were analyzed for CAT activity ... constructs and directions of the promoter in promoter and schematic presentation of the bi-directional different The the The schematic presentation of the bi-directional promoter and the parts and directions...

Ngày tải lên: 12/08/2014, 04:21

... plates and treated with ngặmL)1 IL-1a in the presence and absence of the SC-514 inhibitor (D, E) Time course plot of nuclear NF-jB from in vitro and in silico analysis of the data The plot shows nuclear ... (rhIKK2) and Nandini Kishore for providing the SC-514 inhibitor We thank also Paul Hayter, Sasha Sreckovic and Matthew Strawbridge with help in growing and analyzing the A549 cells and nally ... trichloroacetic acid and the plot shows the number of repeats Phosphorylation of tagged IjBa (d; A) and autophosphorylated rhIKK2 (j; B) is shown In (A), s is the control assay, and in (B), s and D represent...

Ngày tải lên: 16/03/2014, 11:20

... recognized by other receptors For example, vertebrate steroid hormone receptors (such as GRs, mineralcorticoid receptors, progesterone receptors and androgen Arthropod nuclear receptors receptors) bind ... ligand recognition and (c) cofactor interactions The 3D structure of the LBD was first analyzed for RAR [128] and RXR [129], followed by other nuclear receptors The crystal structure of nuclear receptors ... hormone receptors (TRs), retinoic acid receptors (RARs), vitamin D receptors (VDRs) and peroxisome proliferator-activated receptors (PPARs) in mammals The second family, NR2, contains RXRs and hepatocyte...

Ngày tải lên: 23/03/2014, 04:20

... vector and (a) nuclear factor- κB (NF-κB) or (b) retinoic acid response element (RARE) reporter vectors Cells were then treated with or without tumour necrosis factor (TNF)-α (30 ng/ml) and/ or ... protein and type II collagen expression in chondrocytes: Role of MEK1/2 and NFkB signaling pathways J Cell Physiol 2003, 197:356-369 14 Klooster AR, Bernier SM: Tumor necrosis factor α and epidermal ... Preparation of nuclear extracts, immunoblotting, and electrophoretic mobility shift assays Confluent cultures were serum-deprived overnight before addition of TNF-α and/ or atRA for 24 hours Nuclear...

Ngày tải lên: 09/08/2014, 10:22

... the NF-kappa B transcription factor and HIV-1 EMBO J 10, 2247–2258 Shi C, Zhao X, Wang X & Andersson R (2005) Role of nuclear factor- kappaB, reactive oxygen species and cellular signaling in the ... levels of interleukin (IL)-1b and IL-6 in the SP began to rise at h, and reached their peak levels between 48 and 96 h Over the dose range of 0.2–5.0 ngÆmL)1 of SEC1, IL-1b and IL-6 in the SP displayed ... SP and attenuated the febrile response in rabbits (Table 2) Apocynin also inhibited the SEC1-induced nuclear NF-jB expression (Fig 2C, lanes and 4) and its DNA-binding activity (Fig 2D, lanes and...

Ngày tải lên: 19/02/2014, 00:20

... cinerea and S cerevisiae The KER and ED domains are represented by black and grey boxes, respectively (B) Schematic diagram of the truncation mutants of CcCac1L (C) Interaction between CcCac1L and ... Kobayashi R, Kessler N & Stillman B (1995) The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication Cell 81, 1105–1114 Kaufman ... prekaryogamy S-phase and the postkaryogamy DNA replication in C lagopus J Cell Sci 17, 461–470 35 Allers T & Lichten M (2001) Differential timing and control of noncrossover and crossover recombination...

Ngày tải lên: 07/03/2014, 05:20

... by P2Y receptors through signaling pathways involving activation of the transcription factor nuclear factor jB1 (NF-jB1) and the MAPKs extracellular signal-related kinase ⁄ (ERK1 ⁄ 2) and p38 ... and the metabotropic, G protein-coupled P2Y receptors [1–3] Seven subtypes of the P2X receptors (P2X1)7) and eight subtypes of the P2Y receptors (P2Y1,2,4,6,11,12,13,14) have been identified and ... P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12 and P2Y13 receptors and the P2X receptors P2X3, P2X4 and P2X7 in LXF-289 cells PCR products were separated in a 1% agarose gel and visualized with ethidium bromide...

Ngày tải lên: 07/03/2014, 12:20

... establishing cross- talk between these two important transcription factors in the testis [12] Studies have established that TNF-a is a major cytokine produced and released by germ cells and that TNF-a receptors ... dominant role of jB However, cross- talk between NF-jB and HIF-1 in Rnf33 transcription cannot be ruled out because HIF-1a is also a target gene of NF-jB [11,12] On the other hand, FEBS Journal 278 (2011) ... the p65 and p50 NF-jB subunit proteins in testicular cells (A) RT-PCR detection of p50 and p65 transcripts in the testis (Te) and in TM3 and TM4 cells (B) Western blot analysis of the p50 and p65...

Ngày tải lên: 29/03/2014, 00:20

... transcription factors, YB-1, PML and p53, and enhances the up-regulation of the p21(WAFI ⁄ CIPI) promoter by p53 [17] and (b) CARP can bind to DNA [19] and is a negative regulator of the transcription factor ... sequences are localized between amino acids 65 and 88 and the cleavage site is localized before amino acid 71, the region of cleavage would be between amino acids 65 and 71 Considering the location ... transcription factor YB1 in the heart [21] Considering that (a) a molecular complex encompasses calpain and CARP in the N2A elastic region [18]; (b) exercise stimulates both calpain activity [44] and CARP...

Ngày tải lên: 29/03/2014, 21:20

... growth factor (Clontech, Mountain View, CA, USA) and antibiotics Endothelial cells were identified by the characteristic monolayer cobblestone appearance and positive staining for von Willebrand factor ... combination of AGEs and LPS demonstrates a synergistic effect on proinflammatory cytokine ⁄ chemokine production, which requires both mitogen-activated protein (MAP) kinase and nuclear factor- jB (NF-jB) ... AGE-HSA and LPS amplified inflammatory response Furthermore, blockade of both MAP kinases and NF-jB with a combination of inhibitors completely abolished AGE-HSA and LPS costimulated IL-6, IL-8 and...

Ngày tải lên: 30/03/2014, 01:20

... fundamental role of the RGD-mediated interaction between integrins and their ligands for the activation of essential signalling pathways in cell proliferation and growth has been well studied [11] In ... Interaction between cardosin A and the C2 domain of PLDa is mediated through the RGD ⁄ KGE sequence motifs (A) Recombinant wild-type cardosin A (lane 1) and several mutants where the RGD and KGE ... showed that, in both cases, the purified high -molecular- mass protein cross- reacts with the PLD antibody The specificity of the interaction between cardosin A and 5792 I Simoes et al ˜ PLDa was further...

Ngày tải lên: 30/03/2014, 11:20

... 271) 3743 Fig Interaction between p65 and RHA (A) Schematic illustrations of various functional domains of p65 and RHA dsRBD, double stranded RNA-binding domain; NLS, nuclear localization signal; ... medium lacking His, Leu and Trp, and containing 25 mM 3-aminotriazole (Fig 1B) pGBT-p65(1–286), pGBT-p65(286–442), and Table Yeast two–hybrid interaction assays between p65 and RHA Yeast Y190 cells ... Leu, and His, and containing 25 mM 3-aminotriazole Plasmids were rescued from clones that were positive for b-galactosidase activity and identified by nucleotide sequencing cDNA sequences and their...

Ngày tải lên: 30/03/2014, 15:20