... Culture for Studying Heart Development Calvin T Hang and Ching-Pin Chang Quantifying Cardiac Functions in Embryonic and Adult Zebrafish Tiffany Hoage, Yonghe Ding, and Xiaolei Xu ... plunger and inject resin into the heart It is important to steady the syringe and needle while injecting the resin, particularly for smaller embryonic hearts Additional movement of the bevel within ... molecular biology and new available equipments and techniques, and we begin to understand the molecular pathways and cellular interaction in the process of heart induction, rightward looping, chamber...
Ngày tải lên: 12/02/2014, 16:20
... counterstain Incubate cells in 0.2 µg/mL DAPI, and rinse once in PBS To label fixed samples using both TUNEL and immunofluorescence staining for an antigen of interest, the antibody incubations ... fluorescent staining of cultured cells and tissues is presented thereby allowing investigators to utilize the TUNEL staining procedure with a minimal investment in laboratory reagents and equipment In addition, ... DNA making it ideal for labeling fragments in apoptotic cells The enzyme will also label single stranded DNA molecules containing a 3'-OH and will attach nucleotides to a single-strand nick in DNA...
Ngày tải lên: 11/04/2014, 09:47
methods in molecular biology, v.474. nanostructure design. methods and protocols, 2008, p.268
... Protocols and Bioinformatics, edited by Svetlana Gerdes and Andrei L Osterman, 2008 415 Innate Immunity, edited by Jonathan Ewbank and Eric Vivier, 2007 414 Apoptosis in Cancer: Methods and Protocols, ... enabling gene therapy and tissue engineering applications (2022) 1.2 Methodology for Creating and Studying Chimeric Proteins Between Fibritin and Triple-Stranded Segments of Fibrous Proteins The ... creating and studying chimeric proteins was first developed from fundamental studies aimed at structural understanding of fibrous proteins In phage T4 fibritin, a 27-amino acid (aa) domain (amino...
Ngày tải lên: 04/06/2014, 15:06