and its catalytic mechanism is very different

... Kapparanidis A, Oikonomou K, Vasilikos V, Andronikos P, Dermitzakis A, Richter D, Fragakis N, Styliadis I, Mavridis S, EP Stefanadis C, Vardas P: Epidemiological characteristics, management and early ... groups Where CI is cardiac insufficiency, STCS is subacute thrombosis in coronary stent, LT is late thrombosis, RVS is revascularization surgery, CVD is cardiovascular deaths and CVEI is incidence ... angina pectoris (93.2% and 76% respectively) and ECG (96% and 82% respectively) Patients in allopurinol group (n=40) and control group (n=41) received stent implantation and there is no statistical

Ngày tải lên: 19/11/2022, 11:41

... temperature and duration of the synthesis, (v) type of a catalyst of polyreaction and method of its introduction, and (vi) carbonization conditions (heating rate, tempera­ ture, time, and kind of ... 0.60–0.70) is observed (Fig 2A) This shift arises from the shrinkage of the SBA-15 framework caused by the high-temperature treatment (carbonization at 850 � C), what is evident by comparison of the isotherms ... substantially the catalytic performance of the replicas This additionally supports the postulated mechanism of active coke Furthermore, the C-1.25 replica exhibits the lowest selectivity to COx and utmost

Ngày tải lên: 20/12/2022, 21:58

... two-metal-ion catalysis mechanism has been proposed for RNase H [31,46,47]. According to this mechanism, metal ion A is required for substrate-assisted nucleophile formation and product release, and metal ion B is ... active site residues, the histidine residue is well conserved in the type 1 RNase H sequences (His124 for Eco-RNase HI and His539 for HIV-1 RNase H) (Fig. 1). This residue is located in the flexible ... flexible loop near the active site and is involved in the catalytic function, but in an auxiliary manner [23]. This residue is conserved as His264 in human RNase HI, but is replaced by Glu188 in Bha- RNase

Ngày tải lên: 30/03/2014, 02:20

... branch is established by eukaryotic enzymes with unknown biochemical chara- cteristics. In both the ML and N-J trees the b ranch corresponding to aspartylglucosaminidases is clearly dis- tinguishable, ... studies. Acknowledgements We wish to thank Prof. Michal Dadlez, Jacek Oledzki, and Jacek Sikora (Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw) for their help and discussion of ... of the iadA g ene is a zinc isoaspartyldipeptidase [41,42]. However, this enzyme cannot hyd rolyze some of the b-aspartyl dipeptides, and its affinity for b- L -Asp- L -Leu is relatively low (K m ¼

Ngày tải lên: 30/03/2014, 15:20

... or nanoislands. The nanoislands structure could increase surface area of Ag-Pt alloy shells, and improve the Figure 1 (A), SEM image of graphene, (B), EDX analysis of GO, (C), EDX analysis of ... nanoisland/ graphene hybrid composites and its high stability and catalytic activity in methanol electro-oxidation. Nanoscale Research Letters 2011 6:551. Submit your manuscript to a journal and ... oxide is with lowest cost and large scale to prepare graphene, which attract scien- tists’ intensive attention, and exhibit great application prospect. In the field of electrochemistry, graphene is

Ngày tải lên: 20/06/2014, 22:20

... biosynthesis and export When the concentration of simocyclinone and/ or its intermediates is low the transcription of the exporter gene simX is. .. mechanism couples the biosynthesis ... biosynthesis and r esistance genes expression Le et al. 2009. That is why very often resistance genes are linked to antibiotic biosynthesis genes Tahlan et al. 2007; Ostash et al. 2008. As our understanding ... producing organism, the onset of antibiotic production should be under tight control and mechanisms of self- resistance of producing bacteria must exist. All this requires a precise regulatory

Ngày tải lên: 21/06/2014, 17:20

... gammaretroviruses (MoMuLV) and lentiviruses (SIV and HIV) Based on these findings we wanted to study the mechanism by which the Tat mRNA is translated using recombinant monocistronic and bicistronic RNAs ... the colour codes) With this first PCR, the exon1 sense strand partially hybridizes with the exon2 antisense strand and the exon1 antisense strand with the exon2 sense strand Then "Amplification ... cells to sustain its own synthesis To address this issue we studied translation of the Tat mRNA in vitro and in human cells using recombinant monocistronic and dicistronic RNAs containing the 5'

Ngày tải lên: 12/08/2014, 23:22

... infection and the pathogenic... Neil SJ, Sandrin V, Sundquist WI, Bieniasz PD: An interferon-alpha-induced tethering mechanism inhibits HIV-1 and Ebola virus particle release but is counteracted ... [25] and tetherin (BST-2 or CD317) [26,27]. Tetherin is a host transmembrane pro- tein [26,27] and is expressed by a wide-range of human and animal cells [28,29]. Mouse and human pDCs [30,31] and ... maturation and enhances the efficiency of DC- mediated HIV-1 transmission. Type I interferon (IFN) partially inhibits HIV-1 replication and cell-cell transmission in CD4 + T cells and macrophages.

Ngày tải lên: 13/08/2014, 01:20

... data confirm that T20 and P5 exhibit antiviral activities by different mechanism, and that P5 specific activity is calcium dependent. P5 inhibits replication of T20-resistant HIV-1 strains Since ... forward- and side-scatter param- eters. Inhibition of T20-resistant HIV-1 strains by P5 and T20 Two T20-sensitive (pre- Enfurvitide (ENF) and JRCSF) and two T20-resistant strains (ENF-1 and ENF-2) ... P5 and T20, we sought to determine whether P5 is able to inhibit replication of HIV-1 strains resistant to T20. Two T20-sensitive (pre-ENF and JRCSF) and two T20-resistant strains (ENF-1 and

Ngày tải lên: 13/08/2014, 05:21

... ABSTRACT Liyun Cao MECHANISM OF TISSUE TRANSGLUTAMINASE UPREGULATION AND ITS ROLE IN OVARIAN CANCER METASTASIS Ovarian cancer (OC) is a lethal disease due to metastasis and chemoresistance. Our ... purpose of this study was to explore the mechanism by which TG2 is upregulated in OC and its role in OC progression. We demonstrated that transforming growth factor (TGF)-β1 is secreted in ... upregulation and EMT in vitro and tumor dissemination in vivo, which confirms the link between TGF-β1 and TG2 in EMT and tumor metastasis. TG2 expression was correlated with the number and size

Ngày tải lên: 24/08/2014, 10:58

... ligands on NHE1 protein is not drug specific, but is conserved in both the endogenous and synthetic PPARγ ligands.The difference in extent of NHE1 repression by 15d-PGJ2 and synthetic PPARγ ligands ... MDA-MB-231 and T47D were selected to be exposed to various PPARγ ligands at different doses. The three cell lines express different levels of endogenous PPARγ receptor (Figure 2D). Prostaglandin J2 and ... few mismatches in nucleotides. This general pattern of 6-N-6 is relatively conserved in all PPREs, and the identified NHE1 PPRE bears high sequence similarities to these PPREs. In this section,

Ngày tải lên: 09/09/2015, 10:19

... restores its response to E2 on inhibiting PPARγ -mediated down- regulation of NHE1 The status of ERα is a determining factor in the biological behaviors of breast cancers and is closely ... (2X105 cells/6-well dishes), and (B) MDA-MB-2 31 ERα+ (2X10 5 cells/6-well dishes) cells were treated with 15 d-PGJ2 for 24h with or without 2h pre-incubation of 1 M E2, and the protein ... or 15 μM of troglitazone, and the cells were next harvested and subjected to NHE1 mRNA analysis by real-time PCR In cells without E2 treatment, PPARγ ligands brought down the NHE1

Ngày tải lên: 09/09/2015, 10:19

... cell lines. To this end, MCF-7, MDA-MB-231 and T47D cells were treated with different doses of synthetic ligands as well as natural ligand of PPARγ, and the protein expression and mRNA transcript ... inhibition varied from ligand to ligand, this could be explained by the different binding affinities of these ligands to PPARγ receptor. Taken together, we surmise that PPARγ ligands induced downregulation ... O. Braissant, et al. (1995). "Peroxisome proliferator activated receptors: transcriptional regulators of adipogenesis, lipid metabolism and more." Chem Biol 2(5): 261-266. Wanders, R. J. and J.

Ngày tải lên: 09/09/2015, 10:19

... (1. 78 X 10 6 /10 0mm dish) cells were exposed to 1 M... activity and facilitate down- regulation of NHE1 expression by PPARγ ligand 14 7 Figure 23: ERα antagonists enhance PPARγ -mediated ... essential in its inhibitory effect on PPARγ -mediated down- regulation of NHE1, it was hyptotheiszed that ERα and PPARγ compete sterically for DNA binding site in close proximity, and activation ... containing regular serum, and the protein expression of NHE1 was analyzed by Western blot. NHE1 band intensity was normalized to β-actin. (B) MCF-7 (2 X105 cells/6-well dishes) cells were treated

Ngày tải lên: 09/09/2015, 10:19

... AB INITIO AND DFT INVESTIGATION OF THE MECHANISM AND HYDRATION PATTERN OF SIALIDASE AND ITS INHIBITORS KRISHNAN CHANDRASEKARAN NATIONAL UNIVERSITY OF SINGAPORE 2010 AB INITIO AND DFT INVESTIGATION ... INVESTIGATION OF THE MECHANISM AND HYDRATION PATTERN OF SIALIDASE AND ITS INHIBITORS KRISHNAN CHANDRASEKARAN M.S. (By Research) Chemistry National University of Singapore A THESIS SUBMITTED FOR ... Results and Discussion 74 5.2.1 Cluster continuum solvation analysis of DANA-guandino complex 74 5.3 4-Amino-DANA complex 78 5.3.1 Results and discussion 79 5.4 Solvation analysis of 4-guandino-DANA

Ngày tải lên: 11/09/2015, 09:15

... (PKC) and sphingosine kinase (SK) inhibitor, and is currently evaluated in Phase I clinical trials Yet, the underlying mechanisms of its action remain largely unknown The research presented in this ... 0.94) and synergistic in LS-174T cells (CI = 0.68), and resulted in concentration- and time-dependent down-regulation of phosphorylated PKC and its downstream substrate, phosphorylated myristoylated ... Zhan Yuin, Li Fang and Mandy (and the list goes on), who are willing to listen to my latest failed experiment or go for lunch/tea/dinner Thanks to the AAPSNUS Student Chapter and Pharmacy Graduate

Ngày tải lên: 11/09/2015, 10:02

... MAP2 cDNA and its derivatives generated by PCR were cloned into pGEX-4T-2. The KIND domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2. The N- and C-terminal ... hereafter as MAP2) is known to modulate polymerization, stability and rear- rangement of microtubules in neuronal dendrites [13–15] and is associated with some neurological and psychiatric disorders [16,17]. ... substrates and regulatory subunits, implying that the KIND domain, an atypical noncatalytic C-lobe, is involved in the interaction with signaling proteins [3,5,10]. However, the structural and functional...

Ngày tải lên: 14/02/2014, 19:20

... [18]. Scheme 5. The catalytic cycle of LPO and MPO. In this scheme, the role of H 2 O 2 as an oxidant is shown in steps 1 and 2; catalase activity is displayed in step 1;CpdIisomeri- zation is taken into ... LPO and generates Cpd III (with bands at 424, 550 and 588 nm) (Table 2). In contrast to MPO, LPO Cpd III spectral pattern is clearly distinct from that of Cpd II and can be easily distinguished ... describes an integrated vision of the catalytic mechanism of MPO and LPO. A brief survey of the absorption features of MPO, LPO and their intermediates The absorption spectrum of MPO is characterized...

Ngày tải lên: 20/02/2014, 02:21

... in iron metabolism. This includes hepci- din, a 25 amino acid antimicrobial peptide that is expressed in liver cells and is now recognized as a key regulator of iron homeostasis in the body. ... Acton RT (1999) Two novel missense mutations of the HFE gene (I105T and G93R) and identification of the S65C mutation in Alabama hemochromatosis probands. Blood Cells Mol Dis 25, 147–155. 50 Fleming ... differences in intracellular distribution and co-localization of wt- and mutated forms. Blood Cells Mol Dis 39, 75–81. 30 Zhang AS, Davies PS, Carlson HL & Enns CA (2003) Mechanisms of HFE-induced...

Ngày tải lên: 06/03/2014, 22:21