Foundations of Molecular Cloning

... DNA in the middle of a specific base-pair stretch of DNA; one characteristic of certain restriction enzymes is their propensity to cut the DNA substrate in or near specific, often palindromic, ... fundamental studies of the biochemistry of nucleic acids, with particular regard to recombinant DNA.” Transformation Recombinant DNA technology would be severely limited, and molecular...

Ngày tải lên: 09/09/2015, 10:12

... mulitple cloning sites pUC ApR ColE1 origin of replication Lac+, or blue colonies on X-gal in appropriate strains of E coli High copy number foreign DNA lacZ pUC recombinant ApR ColE1 ori Lac-, ... to join DNA: homopolymer tails Alternate method to join DNA: linkers Introduction of recombinant DNA into living cells via vectors • Autonomously replicating DNA molecules – (ha...

Ngày tải lên: 13/03/2014, 18:39

... (2008) A novel plant protein disulfide isomerase family homologous to animal P5 – molecular cloning and characterization as a functional protein for folding of soybean seed-storage proteins FEBS J ... both luminal proteins Generally, PDI family proteins play important roles in folding and quality control of nascent polypeptides [25] In soybean cotyledon, lar...

Ngày tải lên: 18/02/2014, 11:20

... MIR-D40 (Sanyo, Osaka, Japan) To amplify the DNA fragments containing a complete x-5 gliadin gene, oligonucleotides, 5¢-AAGTGAGCAATAGTAAACACAAATCAAAC-3¢ and 5¢-CGTTACATTATGCTCCATTGACTAACAACGA TG-3¢, ... on autoradiography film Dot blot immunoassay for inhibition test Expression and purification of recombinant protein Sense (5¢-ATTTCATATGCAACAACAATTCCCCCAGC AACAATCA-3¢) and antis...

Ngày tải lên: 20/02/2014, 01:20

... 4779 Characterization of PDI from Conus marmoreus Z.-Q Wang et al A B Fig Comparison of amino acid sequences of PDIs from C marmoreus and other species (A) Multiple sequence alignment of PDIs from ... Catalysis of protein folding by protein disulfide isomerase and small-molecule mimics Antioxid Redox Signal 5, 413–424 Song JL & Wang CC (1995) Chaperone-like...

Ngày tải lên: 07/03/2014, 05:20

... process of soybean seeds as decreases in the mRNA levels of GmPDIL-1, GmPDIS-1 [26] and GmPDIM [27] were observed during the accumulation of the storage proteins Expression of certain seed storage proteins ... (2008) A novel plant protein disulfide isomerase family homologous to animal P5: molecular cloning and characterization as a functional protein f...

Ngày tải lên: 07/03/2014, 05:20

... (S)-cheilanthifoline, not (S)-nandinine [7,30,31] If we assume that (S)-nandinine is produced in E californica, there may be no need for a catalyst from (S)-nandinine to (S) -stylopine However, nandinine could ... 1029 Stylopine synthase from Eschscholzia californica N Ikezawa et al E californica [27] Indeed, CYP80B1 has been cloned using this strategy, although other P450s invol...

Ngày tải lên: 07/03/2014, 10:20

... L decemlineata (b) the analysis of mRNA expression profile of L decemlineata, EcR and USP, and (c) the measurement of the binding affinity of steroidal and nonsteroidal ecdysone agonists to the ... hormone receptors of L decemlineata agonists for the molecular interaction with the molting hormone receptor Here, we report (a) the determination of pr...

Ngày tải lên: 07/03/2014, 21:20

... control of 30 s at 95 °C, 30 s at 50 °C and at 72 °C with a preheat at 95 °C and a 10 final extension at 72 °C using primers, S1 (5¢-GCAAATGCAACTGGA AGCGG-3¢) and A1 (5¢-ACAGCCTGCTAGCAAAGA GG-3¢) ... (5¢-ACAGCCTGCTAGCAAAGA GG-3¢) for amplification of HRT1, and primers S2 (5¢-GAAGAATCCTCTAAGGATAA-3¢) and A2 (5¢-TA CAAGGATTAATCCCTTGC-3¢) for amplification of HRT2 The PCR prod...

Ngày tải lên: 07/03/2014, 21:20

... Detection of zebrafish SULT1 ST1 and ST2 mRNAs and (B) Western blot analysis of zebrafish SULT1 ST1 protein (A) Detection of zebrafish SULT1 ST1 and ST2 mRNAs in cultured zebrafish cells (lanes and 3) and ... sequence of zebrafish SULT1 ST1 displayed, respectively, 50%, 50%, and 49% identity to those of mouse SULT1C1, rat SULT1A1, and human SULT1A1 STs [22...

Ngày tải lên: 08/03/2014, 02:20

... tatg|gtaag tatg|gtaag agag|gtaag g(t)5aacag|aagaa c(t)4gtatacag|actc a( t)4cag|atcc c(t)4ag|aatc Not in coding region I I II (2929) (986) (1985) (1490) Table Comparison between exon structures in Xenopus ... 2002 Functional analysis of Xenopus MGP gene promoter (Eur J Biochem 269) 1949 GGAAAC-3Â) for amplication of the region from )783 to +33, and (5Â-CCGGAGCTCGAGGG...

Ngày tải lên: 08/03/2014, 10:20

... Similarly, antibody to Rab32 detected only Rab32 (and minor proteolytic fragments) (Fig 7D) [35S]GTP[S] binding by GST Rab31 and GST Rab32 Fig Dependence of GTP[S] binding by GST Rab31 and GST± Rab32 ... of anti -Rab32 antiserum The positions of prestained protein standards are shown on the right Ó FEBS 2002 Bacterial expression of Rab31 and Rab32 GST Rab31, G...

Ngày tải lên: 08/03/2014, 16:20

... glabra GgbAS1 b-amyrin synthase [6], was prepared by PCR using GgbAS1 as a template, Taq DNA polymerase (Takara Shuzo, Kyoto, Japan), the primers 50 -GAAGCATA TCCACTATGAAGATGA-30 and 50 -TGAATACTCCCGTG ... Ebizuka, Y (2001) Cloning and characterization of a cDNA encoding b-amyrin synthase involved in glycyrrhizin and soyasaponin biosynthesis in licorice Biol Phar...

Ngày tải lên: 08/03/2014, 23:20

... Alignment of amino acid residues in (A) nigrocin-2- related peptides from O schmackeri skin, (B) nigrocin-2- related peptides from O grahami skin, and (C,D) identical nigrocin-2- related peptides from ... feature of nigrocin-2 peptides is reminiscent of the glycine ⁄ leucine-rich dermaseptin orthologues from the skins of neotropical phyllomedusine leaf frogs,...

Ngày tải lên: 15/03/2014, 10:20

... characterization of the human VGSC b1B subunit The human b1B subunit is a novel splicing variant of the b1 subunit via alternative intron retention The retained intron encodes a novel extracellular, ... peptide Functional expression of the human b1B subunit with NaV1.2 in Xenopus oocytes To explore the regulatory function of the hum...

Ngày tải lên: 16/03/2014, 23:20