Báo cáo khoa học: "Diagnosis of left ventricular diastolic dysfunction in the setting of acute changes in loading conditions" pdf

... the C-terminal C 5 -domain, catalyzing the intramolecular nucleophilic attack of the L-hOrn 3 a-amino group onto l-haOrn 4 . Taking into account that the synthetases involved in the bio- synthesis ... encoded in the etc gene cluster. The N-terminal C-domain of the NRPS catalyzes the a-N-acetylation of haOrn 1 in cis. Cyclorelease of the assembled tetrapeptide...

Ngày tải lên: 16/02/2014, 09:20

... all the genes involved in the pathway in the same operon; the cell can then respond quickly to changes in the environ- ment by changing the expression of only a few genes and using the protein-synthesizing ... by the second gene in the las operon (Fig. 1). In order to obtain strains with increased PK activity an additional copy of the pyk gene was recombine...

Ngày tải lên: 19/02/2014, 17:20

... pairing between these sequences within the BX-C plays a role in restricting the extent of loop- ing-out domains. Tethering elements Deletion analysis of the Abd-B gene strongly suggests the existence ... difference in the tim- ing of somatic pairing perhaps reflects the difference between the complexities of the regulation of the two systems: a longer time is re...

Ngày tải lên: 20/02/2014, 01:20

... suggesting that the two adenine nucleotides may work in synergy to maintain the resting tone of the bladder. In conclusion, extracellular cADPR enhances the spontaneous release of ATP in the bladder ... preparation. Of particular importance is the finding that exogenous cADPR failed to increase the spontaneous release of ATP in the absence of CD38. In other...

Ngày tải lên: 05/03/2014, 23:20

... according to the method of Nishikimi et al. [23] did not reveal the pres- ence of any fluorescent protein band, thus pointing to the absence of a covalently bound flavin moiety in the recombinant ... Escherichia coli. The recombinant protein contained an engineered enterokinase cleavage site in the junction between the GST tag and the Rv1771 sequence. Upon 3 h of ind...

Ngày tải lên: 07/03/2014, 12:20

... and postpeptides of Gla-containing conotoxins [20]. In addi- tion, the newly identified propeptides contain a putative consensus sequence found in the precursors of Gla-con- taining conotoxins but not in the ... Gla(3)–TxVI: confirmation of the presence of BrTrp. Product ion mass spectrum of the doubly charged ion at m ⁄ z ¼ 660.18. The isotopic distribution of th...

Ngày tải lên: 23/03/2014, 11:20

... for the lack of in vivo binding of Gads to phosphorylated LAT tyrosine 132) and the formation of multiprotein signaling complexes (the reason for the lack of detectable in vivo association of ... biophysical methods. These approaches offer the opportunity to probe the basic mechanism of protein–protein interactions in great detail. First, the affinity of the...

Ngày tải lên: 23/03/2014, 15:21

... do not inhibit calcineurin. Together, these results suggest that neither mitochondria, inhibition of calcineurin nor blockage of P-glycoprotein play a role in the comparable induction of ROS ... staining of P-glycoprotein Cytospins of GLC4 and GLC4 ⁄ P-glycoprotein cells were incubated for 1 min with hematoxylin for staining of nuclei. To assess the localization of P-glyco...

Ngày tải lên: 30/03/2014, 08:20

... concentration of the inhibitor by nonlinear fit of the data from the above negative controls; [I] is the concentration of the inhibitor, IC 50 is the concentration of inhibitor that causes 50% inhibition; ... the chymotrypsin–BPTI inter- action, lysine is a better P 1 residue than valine, isoleucine, or alanine [41]. X-ray analysis showed that, in this case of lysine,...

Ngày tải lên: 30/03/2014, 13:20

... primarily the task of the Development of the training manual will be ongoing over the life of the project. 16 detailed data on major industry players, including firms of different ... sector. • Field investigation in three regions to examine the value chain of the animal feed industry including an assessment of characteristics and costs of productio...

Ngày tải lên: 21/06/2014, 04:20