báo cáo khoa học: "The glutamine synthetase gene family in Populus" ppt

... Principal Component Analysis (PCA) of GS genes and genes involved in lignin biosynthesis and C1 metabolism. The expression profiles of GS genes were examined in silico during wood formation in ... High-level expression of Pinus sylvestris glutamine synthetase in Escherichia coli. Production of polyclonal antibodies against the recombinant protein and expression studies in pine s...

Ngày tải lên: 11/08/2014, 11:21

... chemokines in vitro (Table 2). DPIV in cell biology A number of DPIV-binding proteins have been identi- fied, including adenosine deaminase [78,79], CD45 (protein tyrosine phosphatase) [80], caveolin-1 ... protein; gko, gene knockout; HSC, hepatic stellate cell; IL, interleukin; IP, interferon-c-inducible protein; IRAK-1, IL-1 receptor-associated serine ⁄ threonine kinase I; ITAC, interf...

Ngày tải lên: 06/03/2014, 09:22

... plasmids were introduced in E. coli strains. osiS expression was induced using arabinose. Expression and purification of recombinant proteins A DNA fragment corresponding to the entire coding region of ... recorded before (bold line) and after (discontinued line) adding 0.1 m M of L-cysteine. (B) Protein–protein interactions between OsiS monomers. OsiS–OsiS interactions were detected using a...

Ngày tải lên: 18/02/2014, 04:20

... trithorax (SET) domain-containing protein SUV39H1 creates binding sites for the chromo- domain-containing protein HP1, resulting in the establishment of heterochromatin [3]. In addition, methylation ... Drosophila interband-specific protein Z4 as a DNA-binding zinc-finger protein determining chro- mosomal structure. J Cell Sci 117, 4253–4264. 53 Ebert A, Schotta G, Lein S, Kubicek S, Krauss...

Ngày tải lên: 23/03/2014, 07:20

Ngày tải lên: 07/08/2014, 18:21

... shock genes, whereas mouse HSF1 is a master regulator of these genes. Interestingly, the amino-terminal region of chicken HSF1 containing an alanine-rich sequence and the DNA-binding domain is ... (PDZ) domain-containing protein PDZK3 ⁄ PDZD2 ⁄ PAPIN (plakophilin-related armadillo repeat protein-interacting protein) [61], decreased, but was still observed in HSF1-null MEF cells during heat...

Ngày tải lên: 18/02/2014, 04:20

... aB-crystallin. Reduction-induced aggregation of the insulin B-chain Light scattering due to DTT-induced amorphous aggregation and precipitation of the insulin B-chain commenced after 10 min and reached ... of insulin. A C B Fig. 2. aB-crystallin protects against the DTT-induced aggregation of insulin, and this activity is enhanced by Arg-HCl. Insulin ( , 0.25 mgÆmL )1 ) was incubated at 37 °C...

Ngày tải lên: 18/02/2014, 16:20

... strong, neutralizing action of the cytosolic RNase inhibitor (cRI), a 50-kDa protein containing 16 leu- cine-rich repeat motifs [7,8]. In fact, onconase from Rana pipiens eggs [9], and seminal RNase ... proteins, with high isoelectric point values. Thus we investigated the effects Table 1. Natural and engineered RNases investigated in this study. IC 50 is the RNase concentration producing...

Ngày tải lên: 19/02/2014, 06:20

... (1998) Lectins as chaper- ones in glycoprotein folding. Curr Opin Struct Biol 8, 587–592. 28 Molinari M & Helenius A (2000) Chaperone selection during glycoprotein translocation into the endoplasmic reticulum. ... for 16 h in 50 mm sodium acetate containing 0.5 lL protease inhibitor cocktail (Sigma) at pH 5.5. PNGase F treatment Immunoprecipitates were eluted by boiling for 4 min in...

Ngày tải lên: 19/02/2014, 17:20

... schemes. Finally, we are engaged in a project adapting the LSE for use in language pedagogy – specifically, as a tool assisting language teaching specialists in creating training and testing materials ... currently being documented and packaged up, for an intended open-source release. 2 In addition to continuing linguistic research with the LSE, we are also experimenting with...

Ngày tải lên: 20/02/2014, 15:20