forensic dna profiling protocols

... selected method for isolating DNA from forensic samples, therefore, often influences the ability to success- fully perform DNA analysis. As a result, several DNA extraction methods have been ... Brology, Vol 98 Foretwc DNA frofdmg Protocols Edited by P J Lincoln and J Thomson 0 Humana Press Inc , Totowa, NJ 19 1 Recovery of High-Molecular-Weight DNA from Blood and Forens...

Ngày tải lên: 11/04/2014, 09:41

... using DNase I to nick the DNA and the DNA polymerase I holoenzyme to label the DNA by nick translation gives less reproducible results than the fi ll-in method using Klenow fragment of DNA polymerase ... RNA. Wash the DNA pellet with 70% ethanol and resuspend in 100 µL deoxyribonuclease I (DNase I) digestion buffer. 3.2. DNA Hydrolysis 1. Follow steps 1–3, Subheading 3.1. 2....

Ngày tải lên: 10/04/2014, 22:13

... cleaved /mg protein/h or /µg DNA/ h. 3.5. Fapy -DNA Glycosylase (FPG) Assay 3.5.1. Preparation of FPG Substrate DNA 3.5.1.1. DEPROTEINIZATION OF SUBSTRATE DNA 1. Dissolve CT DNA at 2 mg/mL in TE (up ... software (see Note 9). 2.5. Fapy DNA Glycosylase (FPG) Assay 2.5.1. Preparation of FPG Substrate DNA ( see Note 10) 2.5.1.1. DEPROTEINIZATION OF DNA 1. Calf thymus DNA (see Not...

Ngày tải lên: 10/04/2014, 22:13

... 94 HUMANA PRESS HUMANA PRESS DNA Topology and Enzymes DNA Topology and Enzymes Edited by Mary-Ann Bjornsti and Neil Osheroff DNA TOPOISOMERASE PROTOCOLS DNA TOPOISOMERASE PROTOCOLS 2-D Agarose-Gel ... supercoiled DNA topoisomers migrate faster through agarose in comparison to linear DNA, nicked circular DNA, or relaxed DNA. For example, as shown in Fig. 1, nega- tively super...

Ngày tải lên: 10/04/2014, 23:16

... PRESS HUMANA PRESS Enzymology and Drugs DNA TOPOISOMERASE PROTOCOLS DNA TOPOISOMERASE PROTOCOLS Enzymology and Drugs Edited by Neil Osheroff and Mary-Ann Bjornsti DNA Decatenation 19 2. The total concentration ... Biology, Vol. 95: DNA Topoisomerase Protocols, Part II: Enzymology and Drugs Edited by N. Osheroff and M.A. Bjornsti © Humana Press Inc., Totowa, NJ 2 DNA Topoisomera...

Ngày tải lên: 11/04/2014, 00:21

... involved in DNA repair, a 32 P-labeled DNA probe is prepared so that it contains the DNA lesion or DNA structure of inter- est. To mask the effects of nonspecific DNA binding proteins, the DNA probe is ... bind to specific DNA sequences (1,2). To study DNA repair, EMSAs have been adapted to detect proteins that bind to specific DNA lesions or structures. In an EMSA, protein...

Ngày tải lên: 11/04/2014, 00:59

... DNA to mmi- maze single-stranded Ml3 DNA contammation. Even so, sometimes the mmiprepared RF Ml3 DNA is still contaminated with single- stranded Ml3 DNA, which may confuse the pattern of DNA ... DNA sequence. The protein sequence allowed the design and synthe- sis of an oligonucleotide that would prime in vitro DNA synthesis within the coat protein gene. Furthermore, the der...

Ngày tải lên: 11/04/2014, 09:40

... mhlblt DNase I digestion throughout the DNA fragment, this could be the result of nonspecific interaction with DNA or direct inhibition of the enzyme itself 6 A major problem with using DNase ... would thus cover about 4 bp of DNA However, DNase I has a small loop, important to the binding process, that fits mto the minor groove of DNA, and spans 3-4 bp of DNA (20). Smce the cat...

Ngày tải lên: 11/04/2014, 09:40

... LLC Table of Contents 1 Forensic Science and Criminal Investigations 1.1 Forensic Archeology: A United Kingdom Perspective 1.2 Palynology: A New Tool for the Forensic Investigator 1.3 Forensic Entomology: ... Geographical Profiling 6.5 A Psycho-Geographical Profile of a Series of Unsolved Murders in Raleigh, North Carolina 7 Ethics in Profiling 7.1 The Role of Ethics in Criminal...

Ngày tải lên: 29/03/2014, 04:20

... Molecular Biology TM VOLUME 148 DNA Protein Interactions SECOND EDITION SECOND EDITION Principles and Protocols Principles and Protocols Edited by Tom Moss DNA Protein Interactions POLII TFIIH Filter-Binding ... In the case of a DNA protein complex, the presence of a given DNA- binding protein will cause the DNA to migrate in a characteristic manner, usually more slowly than the...

Ngày tải lên: 08/04/2014, 12:51