e coli, shiga toxin methods and protocols

E. coli Shiga Toxin Methods and Protocols doc

... to cloned stx 1 and stx 2 genes and their respective nucleotide sequences enabled the development of DNA and oligonucleotide probes for the detection of STEC (reviewed in ref. 5). The introduction ... the LEE (locus of enterocyte effacement) pathogenicity island (2,5,7). However, spo- radic cases of HUS have been associated with over 100 different LEE-positive and LEE-negative STEC...

Ngày tải lên: 23/03/2014, 07:20

e. coli, shiga toxin methods and protocols

... rea- sons, STEC detection methods need to be very sensitive and require minimal specimen volumes. Shiga toxigenic E. coli diagnostic methods are based on the detection of the presence of either Stx ... the subtractive hybridization method. Black lines represent the digested tester DNA and grey lines represent the digested driver DNA. The two different adaptor sequences are given in...

Ngày tải lên: 10/04/2014, 23:03