Báo cáo Y học: Structural heterogeneity of pyrimidine/purine-biased DNA sequence analyzed by atomic force microscopy potx

... PRIORITY PAPER Structural heterogeneity of pyrimidine/purine-biased DNA sequence analyzed by atomic force microscopy Mikio Kato 1,2 , Chad J. McAllister 2 , Shingo Hokabe 1 , Nobuyoshi Shimizu 3 and ... solution. Recently, we have successfully characterized the intramo- lecular triplex structure formed in supercoiled DNA within 46 bp of Pyr/Pur mirror symmetry by...

Ngày tải lên: 31/03/2014, 23:20

... sites of chymotrypsin. D-Chymotrypsinogen is a chimera constructed to contain a trypsinogen propeptide instead of the Cys1–Cys122-linked wild-type chymotryp- sinogen peptide. D-Chymotrypsinogen ... size of the major peptide fragments, designated by Roman numbers, in the sequence of chymotrypsinogen. (B) Analysis with SDS/ PAGE of the peptide fragments generated by D-chymotryps...

Ngày tải lên: 22/02/2014, 07:20

... 2-N-(3-O- acyl 4 -acyl 2 )-b-GlcN-(1–6)-2-N-acyl 1 )3-O-acyl 3 -GlcN where acyl 1 ,acyl 2 and acyl 3 are 3-hydroxyhexadecanoic or 3-hy- droxyoctadecanoic acids, acyl 4 is tetradecanoic or (minor) hexadecanoic ... Acyl 1 ,acyl 2 and acyl 3 residues had hydroxy or acyloxy groups at C-3 ( 13 C signals of C-3 at 69.0–72.4 p.p.m.), while acyl 4 had no substituents. The signals of acyl chains c...

Ngày tải lên: 08/03/2014, 09:20

... separation was achieved by a linear gradient of 2–600 m M NaOAc over a time of 70 min. Fractions were analyzed by analytical HPAEC and appropriately com- bined. Desalting was achieved by gelfiltration ... described previously [13]. Preparation of deacylated LPS of E. coli F653 LPS (2.11 g) was de-O-acylated by mild hydrazinolysis (yield 1.425 g) and 902.5 mg were further subj...

Ngày tải lên: 08/03/2014, 09:20

... slight acidity and the product extracted in chloroform. After methylation with diazomethane it was analysed by GLC- MS. The absolute configuration of 2-hydroxy and 3-hydroxy fatty acids was determined by ... were assigned to tetracyl species with two acyloxyacyl amides in which the secondary fatty acids are C12:0 (2-OH) or C12:0. Thus, the hydrolysis of only these two primary fatty aci...

Ngày tải lên: 24/03/2014, 00:21

... stream of air and the mixture of peracetylated derivatives analysed by GC-MS. Absolute configurations were deduced by analysis of the chiral 2-octyl derivatives according to the procedure of Leontein ... ionization energy of 70 eV and a ionizing current of 0.2 mA. Glycosyl-linkage analysis of LPS, was performed accord- ing to the procedure of Sandford [10]. The permethylate...

Ngày tải lên: 24/03/2014, 04:21

... amounts of sialylated Hex3 glycoforms were indicated. b Minor amounts of Hex2 and Hex3 glycoforms lacking acetyl groups or containing a second glycyl group were indicated. c Very minor amounts of ... complete removal of acetate and glycine, con- firming glycine to be e ster-linked. Information on the location of the a cetyl and glycyl groups as well as glycose sequence was provided...

Ngày tải lên: 31/03/2014, 21:21

... translocation is supplied by the hydrolysis of ATP by SecA and by the proton-motive force Correspondence to J. Tommassen, Department of Molecular Microbiology, Utrecht University, Padualaan 8, 3584 ... acid and analyzed by SDS/PAGE and autoradio- graphy. The precursor and mature forms of PhoE are indicated by p and m, respectively. Fig. 5. Processing of (G-10L)prePhoE is...

Ngày tải lên: 08/03/2014, 09:20

... in diverse ways [2]. In general the macrostructure of phyco- bilisomes consists of a core (constituted by allophycocyanin) surrounded by phycocyanin and phycoerythrin (when present) organized in a periferic ... ratio of phycocyanin to phycoerythrin in rods of certain phycobilisomes to improve light harvesting [8]. Furthermore, modulation of the energy levels of the four chemic...

Ngày tải lên: 08/03/2014, 16:20

... Biosynthesis of riboflavin. Enzymatic formatio n of 6, 7-dimethyl-8-ribityllumazine by heavy Fig. 8. Stereo view of the active sites of the S. pombe 6,7-dimethyl-8-ribityllumazine synthase W2 7Y ... X-ray structure of W2 7Y mutant 6,7-dimethyl-8-ribi- tyllumazine synthase – the pentameric assembly. Pentameric assembly of the 6 ,7- dim eth yl- 8-r ibit yl lumazine synthase m uta...

Ngày tải lên: 30/03/2014, 15:20