Eales - immunology for life scientists 2003

. Cataloging-in-Publication Data Eales, Lesley-Jane. Immunology for life scientists / Lesley-Jane Eales. – 2nd ed. p. cm. Includes bibliographical references and index. ISBN 0-4 7 0-8 452 3-6 (cloth. as comprising the non-specific or innate response and the acquired or specific response. The innate Immunology for Life Scientists, Second Edition. Lesley-Jane Eales. & 2003 John Wiley &. xx...

Ngày tải lên: 23/03/2014, 08:32

. rates are applied. For example, optimum flow rate of 1.0mL/min may be employed for a 4.6-mm-i.d. column. A 2.1-mm-i.d. column has optimum flow rate of 0.2 mL/min. For LC/ESI-MS, ESI-MS is compatible. excellent tools for structural characterization of complex molecules. A third type of MS/MS instruments is a hybrid of tandem-in-space and tandem-in-time devices, including the Q-trap (QQ-2D-linear t...

Ngày tải lên: 15/12/2013, 12:15

. atoms in a structure can provide additional information for struc- tural characterization, such as the differentiation between N- or S-oxide for- mation and hydroxylation in drug metabolism studies.. calibration can provide an order-of-magnitude greater mass accuracy than external calibration for the FT-MS analysis. One example is the high-resolution ESI-FT-ICR MS analysis of a mixture of. 14....

Ngày tải lên: 15/12/2013, 12:15

. (Figure 8-2 ). [2]. TYPES OF METHODS 349 Figure 8-1 . Synthesis of TMP. (Reprinted from reference 1, with permission.) Impurity 1 and Impurity 2, were identified as 2,4-diamino- 5-( -4 -ethoxy- 3,5-dimethoxybenzyl). 2,4-diamino- 5-( -4 -ethoxy- 3,5-dimethoxybenzyl) pyrimidine and 2,4-diamino- 5-( 3-bromo-4, 5- dimethoxybenzyl) pyrimidine, respectively (structures shown in Figure 8-3 ), using LC...

Ngày tải lên: 15/12/2013, 12:15

. screening can be per- formed. For method column screening, generally columns with 10-cm or 5-cm × 3.0-mm i.d. could be used that are packed with 3- m particles. Implementa- tion of a column switcher. lot-to-lot reproducibility). The criteria for selec- tion may include that the column is stable for a certain number of column volumes (efficiency, tailing factor, retention time criteria for. com- p...

Ngày tải lên: 15/12/2013, 12:15

. shorter columns for gradient scouting experiments (5 cm × 4.6mm ) packed with 3- m columns or use a high- pressure system (max pressure 15,000 psi) with 10-cm × 2.1-mm, 1. 7- m parti- cles. Use 35–45°C. ionized form or use a 25 mM ammonium hydroxide buffer (pH 10.5) or 25 mM N-methyl pyrrolidine buffer (pH 10.5) to analyze in its neutral form. Use a 10-cm × 3.0-mm column packed with 3- m particl...

Ngày tải lên: 15/12/2013, 12:15

. analyses were conducted on a 1 5- × 4.6-mm, 3- m column. However, the pH scouting analysis could have been performed faster. A 5-cm × 4.6-mm column on a conventional low-pressure HPLC system could. 5-cm or 10-cm × 2.1-mm column, packed with 1. 7- m particles, could have been used in conjunction with an ultrahigh-pressure HPLC system, allowing for further reduction of the analysis time for. examp...

Ngày tải lên: 15/12/2013, 12:15

. can Π Π rL rL r r 2 30 2 46 30 46 0 425 . . . . . -i.d.column -i.d.column -i.d.column 2 -i.d.column 2 == METHOD DEVELOPMENT APPROACHES 425 be performed for further structural elucidation of the impurities. Deuterated experiments can be performed. achieve equivalent retention on a smaller-i.d. column at the same linear velocity is shown in equation ( 8-2 ). For example, if the original method us...

Ngày tải lên: 15/12/2013, 12:15

. categories of problem-solving task: - Information-gap activities, for example, where each person in a pair has only part of the required information 23 - Reasoning -gap activities, for example making. material for these adult beginners is English For Life- Beginner by Tom Hutchinson (2007). The curriculum is structured a priority towards topic-based or theme-based approach. As it is thought fo...

Ngày tải lên: 20/12/2013, 18:25