E coli Shiga Toxin Methods and Protocols doc

Proteogly cans Methods and Protocols doc

... loading buffer was added to 15 m L of each subfragment PCR product and the samples were mixed well. 3. The samples were loaded onto the agarose gel submerged in TAE buffer and electrophoresed at ... subfragment were screened for correct insert size by EcoRI digestion and the 5 ¢ Agg inserts for correct orientation by XbaI digestion. Positive candidates were sequenced to confi rm in...

Ngày tải lên: 05/03/2014, 23:20

Mucins Methods and Protocols doc

... the interface between the external and the internal environment of the organism, and represent the fi rst line of defence of our body. These genes often have an extensive region of repetitive ... boxes represent sequences missing in the human genome sequence. For MUC5AC , the complete genomic sequence is not known; therefore, the sequence and size of the intron from exon 15 to the ta...

Ngày tải lên: 14/03/2014, 22:20