Báo cáo khoa học: Abundance of intrinsic disorder in SV-IV, a multifunctional androgen-dependent protein secreted from rat seminal vesicle pot

... The Authors Journal compilation ª 2008 FEBS Abundance of intrinsic disorder in SV-IV, a multifunctional androgen-dependent protein secreted from rat seminal vesicle Silvia Vilasi and Raffaele Ragone Dipartimento ... rabbit uteroglobin and the rat seminal vesicle sperm binding protein: prediction of structural features of glutamine substrates for transg...

Ngày tải lên: 23/03/2014, 07:20

... Natick, MA, USA) at a flow rate of 5 lLÆmin )1 . Calibration was achieved in the positive ion mode, using denaturated horse heart myoglobin (Sigma). Human sera and ELISA Microplate wells were coated ... (2004) Characterization of us-ms dynamics of proteins using a combined analysis of 15N NMR relaxation and chemi- cal shift: conformational exchange in plastocyanin induced by hi...

Ngày tải lên: 15/03/2014, 09:20

... myristoylated amino terminal 19 amino acids of NAP-22 (myristoyl-GGKLSKKKKGYNVNDEKAK-amide); NAP-22, neuronal axonal membrane protein, also referred to as brain acid soluble protein 1 (BASP1 protein) , ... This transition is better resolved in cooling than in heating scans, since in some cases the heating scans, initiated at 0 °C, had not reached a steady-state baseline in the...

Ngày tải lên: 07/03/2014, 17:20

... consists of an inner linear region of Araf-(1 fi 5) -a- Araf and of branched non-reducing terminal Ara6 motifs: Arafb1 fi 2Arafa1 fi 5 (A rafb1 fi 2Arafa1 fi 3)Arafa1 fi 5Arafa1. About two-thirds of the terminal ... pep- tidoglycan, d-arabinofuranose (Araf)-containing arabi- nogalactan and arabinomannan polysaccharides, mannans, glucans, long-chain (C 70 –C 90 ) a- branched, b-hydroxy fat...

Ngày tải lên: 30/03/2014, 04:20

... expression and activity of CYP2E1 were downregulated in a rat hepatoma cell line after the administration of proinflammatory cytokines, leading to a loss of catalytic activity. This downregulation was at ... significantly at 24 h after CLP and this increase was attenuated by GdCl 3 . The malondialdehyde (MDA) level in CLP rats was 1.8-fold that of sham-operated rats. The incre...

Ngày tải lên: 14/02/2014, 18:20

... Horse- radish peroxidase-conjugated antibody against caspase-3 (#610325) was from BD Pharmigen, and antibody against b-actin (A5 441) was from Sigma. Statistical analysis All data are expressed as ... As shown in Fig. 4A, increased phosphorylation of eukaryotic translation initiation factor 2a (eIF 2a) was apparent after 16 h of stearate treatment, reached a peak at 22 h, and dec...

Ngày tải lên: 14/02/2014, 22:20

... sites of PTMs and for both identifying and characterizing intact proteins in mix- tures on a chromatographic timescale. Identification of PTMs One of the first research applications demonstrating the ... intact protein analyses, and with ongoing advance- ments in protein chromatographic fractionation, ETD can be extended to larger proteins and protein com- plexes. ETD is a p...

Ngày tải lên: 18/02/2014, 16:20

... 5¢-GTAGGCATGAGAACGGGA AG-3 and for reverse 5¢-GGGGGTAAGAGGAGGAGA AA-3¢ and for CERK-negative forward 5¢-CCGCAAG AGGCTTTATTGTC-3 and reverse 5¢-TATGCCAAGGA CACGGAGAT-3¢, as a negative control ... MEBCYTO Apoptosis Kit was purchased from Medical and Biological Laboratories (Nagoya, Japan), and a Nuclear Extract Kit was from Active Motif (Carlsbad, CA, USA). The ChIP Assay Kit was also...

Ngày tải lên: 18/02/2014, 18:20

... chromatography, with water elution. Control samples were incubated in the absence of NEM. Protein concentration after PD-10 chromatography was determined by the BCA assay, using BSA standards (Pierce). Free ... N-Ac-Trp-OMe) and peroxide levels assayed at the indicated times using a modified FOX assay. (¤) Catalase added; (h) no catalase added. Initial peroxide concentrations were in...

Ngày tải lên: 21/02/2014, 03:20

... Engineering 4-12-1 Nakanarusawa Hitachi, Ibaraki, 316-8511, JAPAN shinnou@lily, dse. ibaraki, ac. jp Abstract In this paper, we propose a practical method to detect Japanese homophone errors in ... The meaning of '~,' is a general will, and the meaning of '~:~'.~.,, is a strong positive will. '~.~.' and '~' have a same phone &ap...

Ngày tải lên: 22/02/2014, 03:20