Báo cáo khoa học: Relationships between the ethanol utilization (alc ) pathway and unrelated catabolic pathways in Aspergillus nidulans pptx

... if L -isoleucine and L -valine are degraded in A. nidulans as they are in yeast, then the formation and conversion of the Fig. 2. D -Galacturonic acid provokes induction of the alc genes. (A) Northern ... of the alcA and alcR genes (NI *) [13]. (B) Putrescine-induced transcription of the alcA and gabA genes in the leaky putrescine utilization mutant punA11 com...

Ngày tải lên: 17/03/2014, 10:20

... m M ) was present from the b eginning of the r econstitution or was added at the e nd of the burst phase (5 min). The yield was the same in both experiments within t he experimental error. The ... incubated in the resolution buffer at 30 °C using protein concentrations in the range 0.5–2.0 m gÆmL )1 until the residual activity w as between 1.5 and 2 .5% o...

Ngày tải lên: 19/02/2014, 16:20

... iNOS FEBS Journal 272 (200 5) 3172–3183 ª 2005 FEBS 3177 binding of an N-hydroxyguanidine moiety in the iNOS heme pocket thus roughly involves the same binding energy as binding of the guanidine ... pharmacological interest [8,9]. Apart from some equilibrium and kinetic constants related to the binding of l-Arg [10–14] and NOHA [10], nothing is known about the thermodynamic...

Ngày tải lên: 16/03/2014, 22:20

... (199 7) Specific inhibition of thrombin-induced cell activation by the neutrophil proteinases elastase, cathepsin G, and pro- teinase 3: evidence for distinct cleavage sites within the aminoterminal ... 2) and ABZ- GIATFRMLMPEQ-EDDnp (substrate 3) were similar in the 2 · 10 2 mm )1 Æs )1 range (Table 1), indicating that the Lys40 in chymase does not act as a...

Ngày tải lên: 28/03/2014, 22:20

... of the MBD1 gene was induced by interferon, indicating that these genes are co-ordinately regulated. The interaction was confirmed in vitro and in vivo and was mapped to the ubiquitin-like domain ... Translation of the methyl CpG-binding protein 1 splice variant 6 (MBD1v 6). The methyl CpG-binding domain (MBD or TAM), Zn-finger domains (CxxC1- 3), nuclear local- ization si...

Ngày tải lên: 30/03/2014, 13:20

... interacting with the en- vironment and observing the resulting feedback, our method automatically learns both the mapping between the text and the commands, and the un- derlying model of the environment. ... task. Instead, we use the training documents to guide the sampling process. During training, we execute the command sequences predicted by the policy functio...

Ngày tải lên: 30/03/2014, 21:20

... (1 1). In this manner, the bacteria could access integrins and inject CagA (1 2). Injected CagA can then induce the massive disruption of cell junctions (1 3) and a loss of cell polarity (1 4). The ... in future stud- ies. Taken together, CagA PY interacts with a surpris- ingly high number of host proteins to induce signalling pathways involved in cell scattering, e...

Ngày tải lên: 14/02/2014, 19:20

... hairpin are involved in formation of the piR- NA-interacting complex (piRC) and function in the transcriptional gene silencing of retrotransposons and genetic elements in germline cells [18]. Investigations ... gener- ation, and to be related to the expression of granuphilin ⁄ slp4 in insulin-producing cells [28]. More- over, miR9 and other neuronal miRs including miR125...

Ngày tải lên: 18/02/2014, 17:20

... IUÆmL )1 ) and penicillin G (100 IUÆmL )1 ), and were incubated (CO 2 -free) at 15 °C. Haemocyte monolayers were then treated for 30 min with culture medium containing bacterial LPS (1 lgÆlL )1 in NaCl ... by incubating sections in 0.3% hydrogen peroxide in methanol for 30 min at room temperature. Slides were then washed and incubated in a blocking solution accor...

Ngày tải lên: 19/02/2014, 00:20

... protein–protein interaction Using the split-ubiquitin system, we investigated the interaction of the C-terminally truncated Alr1 isomers Alr-c36, Alr-c63 and Alr-c96. The protein lacking the very C-terminus of Alr1p ... hetero-oligomeric interactions. Accordingly, we conclude that both the N-terminus and C-terminus of Alr1 and Alr2 are in the same com- partment, i.e. in...

Ngày tải lên: 19/02/2014, 06:20