Báo cáo khoa học: Role of disulfide bonds in goose-type lysozyme potx

... mechanics of noncovalent bonds in polyamino acids. VIII. Cova- lent loops in proteins. Biopolymers 3, 379–399. 52 Goldenberg DP (1985) Dissecting the roles of individual interactions in protein stability: ... consequence of the absence of the two intrachain disulfide bonds. In the case of inverte- brate G-type lysozymes, the high content of Cys residues suggests their...

Ngày tải lên: 16/03/2014, 06:20

... folding reactions of disulfide- containing proteins in vitro [3,4]. Generally, folding reactions of proteins that do not involve disulfide bond formation occur within a few minutes. However, disulfide- containing ... assist protein fold- ing in vitro [15,16]. GSH cleaves unfavorable disulfide bonds (solvent-exposed disulfide bonds) and GSSG promotes the formation of disulfide...

Ngày tải lên: 06/03/2014, 00:21

... not interact with R124 in the ternary complex [20]. Hence, the mechanism of binding of fosfomycin by reinforcement of an initial binding site (mainly K22 and R397) through recruit- ment of secondary ... charged amino acid side chains, R397 and R120, are also involved in fosfomycin binding, and in view of the multitude of interactions, it was assumed that deletion of a sing...

Ngày tải lên: 19/02/2014, 13:20

... periods of times (0–60 min) at 37 °C. The concentration of the biotinylated Ig remaining on the cell surface was then quantified by measuring the binding of [ 125 I]Streptavidin. In the absence of ... They were then incubated with 0.25 mL of incubation medium containing 2 lgÆmL )1 of biotinylated R5T-34 monoclonal Ig [30] at 4 °C for 30 min. In some cases, monensin 40 l M was a...

Ngày tải lên: 21/02/2014, 00:20

... in DptF is GLDSV, indicating that this putative ACP domain is one of the few ACPs in which valine replaces isoleucine (I) or leucine (L) in the conserved sequence. To determine whether DptF is ... remains to be clarified whether all of the fatty acids activated by DptE can be incorpo- rated into the final product or whether there is an interfering specificity of the C III domain of...

Ngày tải lên: 07/03/2014, 04:20

... tributyrin in the presence of increasing concentrations of bile salts (0–6 mm), in the absence or in the presence of colipase. As seen in Fig. 4, increasing the concentration of NaTDC inhib- ited ... proteins was independent of the nature of the C-terminal domain, in contrast to the situation with Nc proteins. Indeed, the C2 domain induced sensitivity of the Nc active...

Ngày tải lên: 16/03/2014, 06:20

... a S1 -casein; CSN1S2, a S2 -casein; CSN2, b-casein; IBSP-II, integrin-binding sialophosphoprotein II; MEPE, matrix extracellular bone phosphoglycoprotein. Potential sites of phosphorylation are shown in ... residue; RBP, riboflavin-binding protein; SAXS, small-angle X-ray scattering; SCPP, secretory calcium-binding phosphoprotein; SP, secreted phosphoprotein; SPP-24, secretory phosphoprote...

Ngày tải lên: 29/03/2014, 23:20

... The amount of intact protein was calculated from the intensity of the protein band. Autoproteolysis in sample solutions containing <20 lgÆmL )1 protein was followed by determining the residual activity ... deceleration of the unfolding process by almost six orders of magnitude after bind- ing of all four calcium ions, corresponding to an increase in Gibbs free energy of act...

Ngày tải lên: 19/02/2014, 17:20

... homogenized in 10 mm Tris ⁄ HCl (pH 8.0) using a sonicator. Each 5 lg of homogenate was incubated with increasing concentrations of trypsin or pro- teinase K at pH 8.0 in a total volume of 20 lL in an ... CA); Lipofectamine Plus Reagent was obtained from Invitrogen (Carlsbad, CA); PI-PLC was obtained from BIOMOL International, L.P. (Plymouth Meeting, PA); aprotinin, doxycycline an...

Ngày tải lên: 16/03/2014, 12:20

... mediator of lethal sepsis, triggering the production of proinflammatory cytokines and liver injury [13]. Therefore, the present study aimed to elucidate the role of KCs in the regulation of CYP isoform ... shown that interactions between KCs and endotoxin comprise the initiating event lead- ing to hepatotoxicity in liver injury, including endotox- emia and ischemia ⁄ reperfusion...

Ngày tải lên: 14/02/2014, 18:20