Báo cáo khoa học: The role of Tyr71 in Streptomyces trypsin on the recognition mechanism of structural protein substrates ppt

... 276 (2009) 5634–5646 ª 2009 The Authors Journal compilation ª 2009 FEBS The role of Tyr71 in Streptomyces trypsin on the recognition mechanism of structural protein substrates Yoshiko Uesugi*, ... we consider that the conformational changes induced by the substitution of residue 71 affect the topological specificity. New insights into substrate recognit...

Ngày tải lên: 16/03/2014, 00:20

... solubilization. It has been reported that inappropriate folding of proteins or inappro- priate assembly of multimeric protein complexes can in uence the surface and overall expression of these proteins in ... dependent on the formation of interdisulphide bonds between CD38 monomers, indicating that the CD38 homodimers in B cells are likely to be stabilized via noncovale...

Ngày tải lên: 23/03/2014, 12:20

... enzymes after only 30 min of incubation, the effect was more pronounced in the case of the L44F mutant. The sharp, single transition with a T m of 64.3 °C, in the melting pro- file of unliganded ... the presence of either PRPP or the purine base alone. These melting profiles suggest that the enzyme is desta- bilized not only in the presence of xanthine and PR...

Ngày tải lên: 16/03/2014, 18:20

... level, the entity-mention model is able to check the gender consistency when considering the active mention “she”. 3.3 Discussion There is an in- focus entity in the condition of the link- ing model ... has three mentions “Mr. Clinton”, “Clinton” and “she”, appear- ing in that order. When considering the mention pair “Clinton” and “she”, the model may tend to link them be...

Ngày tải lên: 31/03/2014, 03:20

... tokens on both sides of the parallel data, with the change in BLEU scores. The graph in Figure 3 plots the BLEU scores and the number of tokens in the two sides of the training data as the data ... links. The representation at the bottom of this figure (except for the co-indexation markers) corresponds to the final transformed form of the parallel tra...

Ngày tải lên: 07/03/2014, 22:20

... post-transfection by maintaining the cells in culture medium containing 2% horse serum. Cells were fixed on day 4 of differentiation and stained with hematoxylin and eosin. One of two independent ... biologically relevant role of miR-27 in the regulation of adipose tis- sue functions in vivo, we examined the expression of miR-27 in the genetically obese ob ⁄ ob mi...

Ngày tải lên: 18/02/2014, 08:20

... thus blocking protein synthesis and leading cells to death. The current model proposed for the mechanism of antitumour action of BS-RNase is based on the ability of the protein to resist the neutralizing ... hypothesize that the cytotoxic activity of the latter monomers was due to the presence in these proteins of disulfide bonds, with their potential chemic...

Ngày tải lên: 20/02/2014, 11:20

... obtained by other groups who also point to the role of the connecting loop of the b-hairpin of defensins. A combination of mutational analysis [35] and structural analysis of the plant defensin Rs-AFP1 ... observations, it is not possible to infer whether the key role of the b-hairpin loop of MGD1 (loop 3) in antimicrobial activity of MGD1 is of general v...

Ngày tải lên: 20/02/2014, 11:20

... with increasing solvent-accessibility of the His210 side-chain. In the protein interior, the dielectrostatic constant is lower than on the protein surface, while the dielectrostatic constant in water ... pH-activity curve of API The pH-activity profiles depending on NaCl concentration were determined as part of the investigation into the shielding from solvent...

Ngày tải lên: 21/02/2014, 03:20

... strongly on the levels of expression of Adxp but not Adrp and therefore that the concentration of Adxp is the major factor controlling pregnenolone synthesis in recombinant yeast. In conclusion, ... in ergosta-5-eneol content than in the pregnenolone accumulating strain CA14/pCD63. This result is consistent with observation of the absence of inhibition by pregnen...

Ngày tải lên: 08/03/2014, 08:20