Báo cáo khoa học: Diverging regulation of pyruvate dehydrogenase kinase isoform gene expression in cultured human muscle cells pot

... counterstaining. Haematoxylin–eosin staining Haematoxylin–eosin staining was carried out following the routine histological method described by Kayser and Bubenzer [38]. The haematoxylin–eosin staining technique employs ... absorptive site of folic acid, i.e. the small intestine. Expression of the RFC protein in BBM of the intestine The effect of chronic alcoholism on the level o...

Ngày tải lên: 18/02/2014, 16:20

... depending on the exact location of the AB CD EF Fig. 2. Oxidation-induced conformational changes in the intracellular domains of RPTPs. GST-fusion proteins encoding the intracellular domain of (A) ... hemagglutinin; LAR, leukocyte common antigen related; PTK, protein-tyrosine kinase; PTP, protein tyrosine phosphatase; PVDF, poly(vinylidene difluoride); RPTP, receptor protein-tyrosine...

Ngày tải lên: 18/02/2014, 17:20

... short interfering RNA (siRNA) in human cells. Both HO-2 mRNA and protein are expressed in the eight human cancer cell lines examined, and HO-1 expression is detectable in five of the cell lines, including ... may in uence the expression of HO-1. The dominant expression of HO-2 protein, in comparison with the low expression of HO-1 protein, in H146 small cell lun...

Ngày tải lên: 19/02/2014, 05:20

... measurements of the P P P P P P Import, ExportA B C DNA binding Concerted regulation P P P P P P Import Export DNA binding Modular regulation P P P Import DNA binding Export Individual regulation Fig. ... obtained in special cases. The order of phos- phate processing and the characteristics of protein–protein interactions turn out to be of overriding importance for both sensit...

Ngày tải lên: 07/03/2014, 10:20

... enrichment in iNOS activity within the particulate fraction of epithelial cells. Apical distribution of iNOS in intestinal epithelial cells To get more insight into the localization of iNOS in intestinal ... to apical domains of intestinal epithe- lial cells. Particulate fraction association of iNOS in vivo In order to determine the distribution of iNOS in intest...

Ngày tải lên: 19/02/2014, 16:20

... 4.9 a This strain was grown in media lacking leucine. The other strains were grown in media containing leucine. Fig. 5. Complementation of pos5 cell. Indi- cated pos5 and WT cells carrying each gene on ... quantitation of microgram quantities of protein util- izing the principle of protein dye-binding. Anal Biochem 72, 248–254. 33 Laemmli UK (1970) Cleavage of structural pro...

Ngày tải lên: 20/02/2014, 01:20

... realizing topic continuity in a dialogue. Since new topics are introduced at the beginning of new subdialogues and developed in the following sentences, one can predict that pronominal noun ... STRUCTURES 227 DISTRIBUTION OF NON-PRONOMINAL AND PRONOMINAL NOUN PHRASES Non-pronominal noun phrases play a role in indicating and realizing topic shifts in a dialogue. Since new s...

Ngày tải lên: 21/02/2014, 20:20

... peripheral infection, a huge body of evi- dence points to the role of immune cells in the neuro- invasion process [15–18]. Transport through epithelial colon cells in the presence of differentiated M -cells may ... dependent on the prion strain used for infection [33,34]. Microglial cells can contain infectivity in vivo and may disseminate prion infectivity within the brain...

Ngày tải lên: 07/03/2014, 05:20

... post-transfection by maintaining the cells in culture medium containing 2% horse serum. Cells were fixed on day 4 of differentiation and stained with hematoxylin and eosin. One of two independent experiments ... biologically relevant role of miR-27 in the regulation of adipose tis- sue functions in vivo, we examined the expression of miR-27 in the genetically obese ob...

Ngày tải lên: 18/02/2014, 08:20

... the C-terminal region, including the PAS-B domain of BMAL1, is required for the binding to DEC1, Fig. 4. Identification of the domain of D EC1 that interacts with BMAL 1. Interactions of various ... changes in the expression of liver genes, including several clock-controlled genes, although n o change was observed in clock gene expression in the liver. DEC1 is thus confirm...

Ngày tải lên: 19/02/2014, 16:20