Báo cáo khoa học: Structure of Streptococcus agalactiae serine⁄threonine phosphatase The subdomain conformation is coupled to the binding of a third metal ion pptx

... a third metal ion. The implications of the M3 binding and flap subdomain conformations to the catalytic mechanism are discussed below. The role of the third metal in catalysis In MtSTP [9], Ser160 takes ... PP2C enzymes may also have additional domains, for exam- ple, Arabidopsis thaliana ABI1 in which the catalytic domain is fused to an EF-hand motif, and...

Ngày tải lên: 07/03/2014, 09:20

... by MARs and may explain why MARs often exist along- side enhancers. Chromatin structure regulation by histone acetylation The role of histone acetylation Histone modifications help to create a more ... might acetylate active genes in association with the elongating Pol II. In addition to yeast SAGA and NuA4, the mammalian HAT HBO1 is also potentially able to be recruited t...

Ngày tải lên: 14/02/2014, 18:20

... nucleotide. Discussion The goal of the present work was to better understand the catalytic mechanism of RNase Sa2 and to account for the differences in catalytic activity between RNases Sa2 and Sa. On the basis of the ... GI, Moiseyev GP, Struminskaya NK, Bor- zykh OA, Kipenskaya LV, Znamenskaya LV, LEschins- kaya IB, Chernokalskaya EB & Hartley B (1994) Mutational a...

Ngày tải lên: 18/02/2014, 11:20

... Tetrasialyl PA glycan Neu5Aca2-6Galb1-4GlcNAcb1-2Mana1-6(Neu5- Aca2-3Galb1-3(Neu5Aca2-6)GlcNAcb1-4(Neu5Aca2-6Galb1- 4GlcNAcb1-2)Ma na1-3)Manb1-4GlcNAcb1-4GlcNA-PA was used as a control sugar to ... PA-oligosaccharides, GalNAca1-3(Fuca1-2)Galb1-3(Fuca1-4)GlcNAcb1-3Galb1- 4Glc-PA and Neu5Aca2-6Galb1-4GlcNAcb1-2Mana1- 6(Neu5Aca2-3Galb1-3(Neu5Aca2-6)GlcNAcb1-4(Neu5Aca2- 6Galb1-4GlcNAcb1-2)Man...

Ngày tải lên: 18/02/2014, 14:20

... on their evolutionary relationship and tertiary structure. Plant APs have been distributed among families A1 , A3 , A1 1 and A1 2 of clan AA, and family A2 2 of clan AD. The majority of plant APs ... formation of a two-chain enzyme, without the prosegment and the PSI domain, with a domain organization similar to that of mammalian or microbial APs. An inactivation m...

Ngày tải lên: 19/02/2014, 12:20

... (2-amino- D , L -dodecanoic acid, Laa) to either the N-terminus (LaaMII) or to Asn5 (5LaaMII). The N-terminal LaaMII was shown to have a tertiary structure similar to that of the native conotoxin and maintained the activity ... Mutational analyses have also been valuable in elucidating factors important for activity. Table 2 lists the most informative mutations that ha...

Ngày tải lên: 19/02/2014, 12:20

... Ding-Kwo Chang Institute of Chemistry, Academia Sinica, Taipei, Taiwan, Republic of China The structure and membrane interaction of the internal fusion peptide (IFP) fragment of the avian sarcoma and leucosis ... revent the FP from immersing too d eeply into the apolar core of the membrane. Our data are also compatible with the observation that truncation of t he m...

Ngày tải lên: 19/02/2014, 16:20

... crystallo- graphic asymmetric units make the same contacts around the twofold axis a s i n CG2 (Fig. 1A) . These consist m ainly Table 1. Crystallographic data. Data collection a nd phasing statistics. Data ... chain carboxylate group of the Asp80 residue are within hydrogen bond distance to the Nd1atomofHis78 (3.0 and 3.3 A Ê , respectively). The second of the metal...

Ngày tải lên: 21/02/2014, 03:20

... 13599–13604. 13 Takeda K, Iizuka M, Watanabe T, Nakagawa J, Kawasaki S & Niimura Y (2007) Synechocystis DrgA protein functioning as nitroreductase and ferric reductase is capable of catalyzing the Fenton ... calculated by linear regression analysis of the logarithms of the concentration ratios for oxidized and reduced forms of the mediator versus that of recombinant fla...

Ngày tải lên: 07/03/2014, 02:20

... transmembrane domain, and an intracellular domain consisting of an ATP -binding regulatory domain and a GCase catalytic domain [8]. ANP binding to the ECD stimulates the intracellular GCase domain, thereby ... quaternary arrangement of the ECD dimer without significant intramolecular structure change. This change in the quaternary structure causes an alteration in the...

Ngày tải lên: 07/03/2014, 03:20