Tài liệu Báo cáo Y học: Evolution of the enzymes of the citric acid cycle and the glyoxylate cycle of higher plants pdf
... individual enzymes. However, the study of the enzymes of the tricarboxylic acid cycle necessarily also entails the s tudy of the several enzymes involved in t he glyoxylate cycle in plants, because three ... the tricarboxylic acid cycle (Eur. J. Biochem. 269) 883 Evolution of the enzymes of the citric acid cycle and the glyoxylate...
Ngày tải lên: 22/02/2014, 04:20
... course of storage starch synthesis show that amylopectin and amylose synthesis are partly disconnected and that amylose synthesis persists when the rate of polysaccharide and amylopectin synthesis ... followed the kinetics of amylose synthesis over a 5-day period of nitrogen starvation and measured the amounts of starch, amylose, the k max of the starch fractions...
Ngày tải lên: 22/02/2014, 07:20
... The phosphoryl and pyridoxyl moieties of the P-pyridoxyl group are shown in red and magenta, respectively. The fractional solvent exposed area of the P-pyridoxyl group is 0.39 and 0.082 for the ... another substrate of the enzyme, also increased the P-pyridoxyl fluorescence. The addition of this substrate to the protein blue shifted the maximum by 5 nm and...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
... were recently introduced for the preparation of C-6 aldehyde derivatives of Glc [15]. The key step is the use of Collins reagent for the selective oxidation of the primary trimethyl- silyl ether of the ... G. (1986) The oxidation of primary trimethylsilyl ethers to aldehydes: a selective conversion of a primary hydroxy group into an aldehyde group in the presence...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Dnmt3a and Dnmt1 functionally cooperate during de novo methylation of DNA pdf
... methyl groups are always introduced, one slowly by Dnmt3a or by de novo methylation catalyzed by Dnmt1 and the second one very fast. Therefore, this effect can only double the rate of DNA methylation, ... mainten- ance of the methylation at certain DNA sequences. A role of Fig. 3. Stimulation of Dnmt1 and Dnmt3a by fully methylated CpG sites. In this experiment the rates...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... 2 h and 20 h to determine the substrate specificity of the enzyme. The degradation of the polymer and subsequent oligosaccharide formation was analyzed by HPAEC and MALDI-TOF MS. HPAEC analysis ... both bacteria and fungi [11–15]. Based on their derived amino acid sequence, the corresponding enzymes have been assigned to family 53 of the glycosyl hydrolases [16]....
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... governed by one or even two residues, but is most likely accomplished by the concerted effects of a combination of key residues. Further analysis of these and other mutant SODs is currently underway. ACKNOWLEDGEMENTS We ... represents either iron or manganese. Selectivity of the proteins for their metal cofactor has been demonstrated in vivo [3] and although apoenzymes of ea...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168 pdf
... characterization of specific enzymes that are involved in the biosynthesis of these structures and may lead to the discovery of potential therapeutic targets. In addition, the correlation of carbohydrate ... established by NMR was observed in the FAB- MS and MALDI-MS (Fig. 8) of the methylated PS-2. The MALDI-MS spectra shows a series of A-type primary glycosyl ox...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Soluble silk-like organic matrix in the nacreous layer of the bivalve Pinctada maxima A new insight in the biomineralization field pptx
... was very similar to the WSM, and consequently the EDTA-IM. Hydroxyproline, hydroxy- lysine and phosphoserine were not detected in WSM, WIM, EDTA-SM and EDTA-IM. The comparison of the amino acid ... for the first time the results of a comparative study on the organic matrix extracted from the nacreous layer of the shell from the pearl oyster Pinctada maxima by...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Inactivation of the 2-oxo acid dehydrogenase complexes upon generation of intrinsic radical species pptx
... appearance of the ThDP adduct with the substrate moiety during the complex-catalyzed succinyl-CoA hydro- lysis [72] accounts for the inactivation by dihydrolipoamide in the presence of succinyl-CoA ... 1e – oxidation of the carbanion in the E1 active site (a product of reaction 1) by highly electrophilic thiyl radical of the dihydrolipoyl residue of E2 had to be ex...
Ngày tải lên: 21/02/2014, 01:21