Tài liệu Báo cáo khoa học: Localization of N-linked carbohydrate chains in glycoprotein ZPA of the bovine egg zona pellucida pptx

... to the string including errors from the String- Database (the former string is referred to as the Similar-String, and the latter as the Error-String). Finally, the correction is made using the ... 1245(-8.5) The values inside brackets 0 are the rate of decrease In EPC+SSC, the rate of decrease was 8.5%, and the decrease was obtained in all type of errors....

Ngày tải lên: 20/02/2014, 18:20

... glycosylated. Determination of the number of N-glycosylation sites We then investigated the number of N-glycosylation sites and the localization of N-linked chains in ZPA using the purified ZPA. In the 15% gel, the ... mapping on HPLC referring to the reported structures of the unfractionated zona protein mixture [14]. To obtain the core carbohydrate...

Ngày tải lên: 21/02/2014, 03:20

... promoting an increase in proopiomelanocortin and cocaine and amphetamine regulated transcript. Taken together, these findings provide a compelling argument for the role for malo- nyl-CoA in regulating ... and certain amino acids [16]. A linkage between fatty acid synthesis in the CNS and feeding behavior was uncovered with the finding that systemic or intracerebroventricular adminis...

Ngày tải lên: 14/02/2014, 22:20

... conformation, the SH2 domain binds to pY i on the C-terminal tail, and the Src homology 3 (SH3) domain binds to the linker between the SH2 and kinase domains at the back of the small lobe, preventing the ... by presenting the pulse of Src activity in the plane of the inactive and active fractions, s i and s 1 . If the duration of the stimulus exceeds the...

Ngày tải lên: 18/02/2014, 11:20

... of rhodamine dyes into cells is to follow their quenching upon entering the cells. In respiring cells, the active concentration of these dyes in the mitochondria results in quenching of their fluores- cence. ... allows the estimation of the effect of intracellular binding on the MDR phenomenon. The data detailed obtained in the present study indicate that inna...

Ngày tải lên: 18/02/2014, 13:20

... composition, indicating that the peptide is of the correct sequence and free of contaminating pro- teins. Five cycles of N-terminal sequencing confirmed the expected residues including the presence of methio- nine ... : 2000 in blocking solution for 30 min. Cells were rinsed in NaCl ⁄ P i several times and incubated in blocking serum containing anti-mouse IgG (Vectastain)...

Ngày tải lên: 18/02/2014, 13:20

... transcriptional initi- ation sites. Determining the translation initiation site of isoform 2 KLK11 Messenger RNA for isoform 2 contains two initiation codons near the 5¢ end. To examine whether the first initiation ... isoform 3 (GenBank accession number AB261897), which contains 25 additional amino acids in the catalytic domain, in the prostate [15]. The expression of such...

Ngày tải lên: 19/02/2014, 06:20

... synthesized in five steps from D -pantothenate. The Dfp flavoprotein catalyzes the synthe- sis of the coenzyme A precursor 4¢-phosphopantetheine from 4¢-phosphopantothenate and cysteine using the ... important for the second-half reaction and are part of the active-site of CoaB; these residues may be directly involved in binding of one of the substrates, the amino...

Ngày tải lên: 19/02/2014, 12:20

... cycles in such a combination do not touch each other). The value (the sign) of a cycle is the product of the values (the signs) of its steps. The sign o f the magnitude of a Ôminus combined autoinfluence ... concentrations of its species. Therewith the c oefficient of o rder p of the characteristic equation equals the sum of all simplest combined Ôminus autoi...

Ngày tải lên: 19/02/2014, 16:20

... lumen [43,44]. Considering the importance of the intestinal barrier in the process of oral prion infection, this find- ing might help clarify the entry and routing of PrP Sc in the early steps of infection. ... dpf in enterocytes of the pos- terior part of the intestine (Fig. 5M,Q,R). This last finding should be of interest because in terms of func- tion,...

Ngày tải lên: 19/02/2014, 16:20