Tài liệu Báo cáo khoa học: The calcium-induced switch in the troponin complex probed by fluorescent mutants of troponin I doc

... cells might contribute to providing chromophore to melanopsin to maintain their light sensitivities. The expression and function of RPE65c in other cell types in the retina remains to be investigated. Earlier ... GS staining (B3), and 4¢,6-diamidino-2-phenylindole (DAPI) staining (B4), respectively. (B5-8) High magnification images of the boxed area in (B4) showing RPE65c stain...

Ngày tải lên: 14/02/2014, 14:20

... metabolism may be increased in parallel. As optimization of glucose metabolism is increasingly highlighted as a therapeutic intervention for ischemia-induced and ischemia–reperfusion-induced cardiac ... (FL-AA); Sigma, St Louis, MO] containing luciferin and luciferase. The reaction of the luciferin ⁄ luciferase mixture, energy donor (ATP) and oxygen results in the emission of...

Ngày tải lên: 18/02/2014, 16:20

... signaling. Similarly, sensing of glutamine-induced hepatocyte swelling by integrins feeds into Src-dependent p38 activation, which is criti- cally required for autophagic proteolysis inhibition ... studies have identified the integrin system as one major sensor of hepatocyte swelling [12–14]. Integrin-inhibitory peptides exhibiting an arginine-gly- cine-aspartic acid (RGD) motif abolis...

Ngày tải lên: 18/02/2014, 16:20

... stiff but instead the ring idles in front of the positive charge. This site is accessible to the outside, and ions (H + ,Na + ) flow from the outside to the binding site (driven by the electrical ... and, like its eukaryotic counter- part, is missing one ion binding site (in hairpin two). The genes atpE 2 and atpE 3 encode two identical 8 kDa subunits with one ion-binding...

Ngày tải lên: 18/02/2014, 17:20

... Follistatin, Chordin and Noggin [12]. These proteins are antagonists of BMP and other members of the TGF-b family, binding to and inhibit- ing these signaling molecules from binding to their receptors ... their receptors in the extracellular space, thus inhibiting ven- tralizing activities. Of these proteins, Chordin has a long-range effect. In Xenopus, expression of the cho...

Ngày tải lên: 19/02/2014, 00:20

... since we identified no other inner membrane proteins in our vesicle pre- paration, we suggest that Gut2 is an outer membrane protein with its N-terminal FAD-binding domain facing the intermembrane ... treated with trypsin. The GFP domain was released with protease, while trypsin- sensitive proteins like cytochrome b 2 (in the mito- A BCD Fig. 2. Identification and characterization of...

Ngày tải lên: 19/02/2014, 07:20

... subunits: troponin C (TnC) has the Ca 2+ - binding sites, troponin I (TnI) has the inhibitory function, and troponin T (TnT) is the actin–tropomyosin-binding component. Tn works as a sensor of intracellular ... properties of isolated TnC are well known. Sites III and IV in the C-domain (carboxy terminal) bind Ca 2+ with higher affinity, while sites I and II in the N...

Ngày tải lên: 20/02/2014, 11:20

... 1957) is a general technique that is used to esti- mate the probability distributions of data. The over-riding principle in ME is that when nothing is known, the distribution should be as uniform ... experiments using our learner corpus, which contains information on learners’ errors. 1 Introduction One of the most important things in keeping up with our current infor...

Ngày tải lên: 20/02/2014, 16:20

... output by MIKE and evaluates it in terms of efficiency of communication. 2 Principles of Content Selection in the Real Time Discourse 2.1 Maximization of Total Information A discourse is most ... of information communicated to the audience. We describe how a principle of maximizing the total gain of importance scores during a game can be used to incorporate con...

Ngày tải lên: 20/02/2014, 18:20

... Their difference is the indicated DpH. F or the lowest DpH differences the Ôacidic Õ solution contained 20 m M Tricine instead of succinic acid. Assuming complete equilibration of the K + during the 1 h preincubation ... injected into the basic medium as indicated by the arrow. The ATP synthesis following chromatophores injection was mo nitored continuously with t he luc...

Ngày tải lên: 21/02/2014, 03:20