Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

... substrate binding sites. In the case of the titin and twitchin kinases, the autoinhibitory sequence acts as a pseudo- substrate, occluding ATP binding and preventing protein substrates from binding ... CaM. However in PhK5 Trp357 is found at the C-terminal end of the peptide. This suggests that either PhK5 might bind with Trp357 in the N-terminal site in CaM, or...

Ngày tải lên: 19/02/2014, 17:20

... structural role in the proper folding and disulfide bonding in insulin [2]. After proinsulin cleavage, it is released to the blood together with insu- lin in equimolar amounts. The 31-residue peptide ... distribu- tion of 100 l M proinsulin C -peptide in the presence of divalent Ca 2+ and Mg 2+ ions under native conditions (B), and of 100 l M C -peptide in the...

Ngày tải lên: 18/02/2014, 04:20

... through the binding of RNA-binding proteins to 3¢ UTR tran- scripts has been poorly described in plants. PUF proteins are a large family of RNA-binding proteins found in all eukaryotes. These proteins ... Scheme of the three-hybrid strategy used in the screen. (B) Number of colonies identified in each step of the screen. (C) Distribution of the 63 distinct sequenc...

Ngày tải lên: 18/02/2014, 06:20

... by xanthine oxidase was followed indirectly by measuring the increase in absorbance at 550 nm resulting from cyto- chrome c being reduced by superoxide in the course of the reaction. The principle of ... Interestingly, the position of the glutamate ligand was not conserved in the amino acid sequence, relative to the cupin core motifs that contribute the three his...

Ngày tải lên: 18/02/2014, 06:20

... only critical for the stability of the dimer, it may also be involved in maintaining the integrity of the active site. These results clearly suggest that the dimer interface in the Y74W* mutant ... the backbone CO of F102. Fur- thermore, the orientation of the side chain of the two residues brings the guanidinium plane and the aro- matic ring of F102 in...

Ngày tải lên: 18/02/2014, 11:20

... to the GBS of the ribozyme for the exon-ligation step of TES. In the substrate-cleavage step, however, the 3¢ terminal guanosine (G336) of the ribozyme (Fig. 1) is binding to that same GBS. Therefore, ... for the second step of TES [48], similar to (if not the same as) the role of the xG in the second step of self-splicing [48–56]. This suggests that the...

Ngày tải lên: 18/02/2014, 18:20

... nm of each free hemin solution was measured, and these were plotted together with that of the corresponding hemin– protein solutions. The equivalent was determined at an in ection point of the ... intersection point corresponding to the mole fraction of 0.5 of the respective forms gives the amount of azide necessary to attain the equilibrium, thereby giving the...

Ngày tải lên: 19/02/2014, 05:20

... November 2006) doi:10.1111/j.1742-4658.2006.05579.x Chemokines elicit their function by binding receptors of the G-protein-cou- pled receptor class, and the N-terminal domain (N-domain) of the receptor is one of the two critical ligand-binding sites. ... in uenced by the choice of buffer if proton transfer accompanies the binding process. In this case, the measured en...

Ngày tải lên: 19/02/2014, 05:20

... using crotonyl-CoA as a substrate [15]. The gene encoding this bifunctional protein was named AUH (‘AU bind- ing homolog of enoyl-CoA hydratase’). The RNA- binding activity of the human protein ... to an HPLC vial. The products of the reaction were detected at 260 nm using the HPLC system described above for the synthesis of the CoA- esters. The assay was linear wit...

Ngày tải lên: 19/02/2014, 07:20

... subtilis protein) in the direct vicinity of the FMN binding site which is not present in the other members of the family. Thus, under structural aspects, Idi-2 appears as a fairly distant relative of the ... of the enzyme for that cofactor and the apparent absence of a redox process as part of the catalytic cycle. The substrate binding site of the type...

Ngày tải lên: 19/02/2014, 13:20