Tài liệu Báo cáo khoa học: Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins pdf

... Graether and B. D. Sykes (Eur. J. Biochem. 271) Ó FEBS 2004 REVIEW ARTICLE Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins Steffen P. Graether ... verlap of the X-ray structure with the 30 °C NMR structure using the main chain of residues Ser12fiThr70 in the structure a lignment. (C) Overlap...

Ngày tải lên: 19/02/2014, 16:20

... com- posed of an extracellular ligand-binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. The binding of a ligand to the extracel- lular domain of the EGFR induces ... phos- phorylating the retinoblastoma (RB) protein. Activa- tion of cyclin–CDK complexes is counterbalanced by CDK inhibitors, including those of the kinase inhibitor protei...

Ngày tải lên: 16/02/2014, 09:20

... [17–19]. Pantothenate kinase is an essential regulatory enzyme in co-enzyme A biosynthesis, catalyzing the cytosolic phosphorylation of pantothenate (vitamin B5), N-pantothenoylcysteine and pantetheine ... destinations. Cells from NPC subjects show a decrease in acid sphingomyelinase activity, leading to the accumulation of sphingomyelin [27]. Because one of the pathways for...

Ngày tải lên: 18/02/2014, 14:20

... essential for their cor- rect folding, trafficking and function [4–8]. The intracellular C-terminal tail of P2X 7 is consider- ably longer than those of other members of the P2X family, and contains multiple ... Caveolin-1 staining pattern at the cell surface, confirming the known caveolar location of Caveolin-1. In addition, intracellularly distinguishable punctate patt...

Ngày tải lên: 19/02/2014, 00:20

... documents). The source of Wik- iXML is the English part of the Wikipedia XML Corpus by Denoyer and Gallinari (2006). In the original data some of the HTML-structures like lists and tables occurred within ... answers each. The total cost of producing the corpus was about $350, consisting of $310 paid in workers rewards and $40 in Mechanical Turk fees, includ...

Ngày tải lên: 20/02/2014, 09:20

... which do not contain an in- put keyword. Since the extraction module (see Sec- tion 2.3) analyzes the usage of the input term in re- trieved pages, pages not containing the term are of no use for ... FDDI, d) token ring In the autumn of 1999, out of 80 questions, the num- ber of the first and second types were 22 and 18, re- spectively. 4.3 Implementing a QA s...

Ngày tải lên: 20/02/2014, 18:20

... monitored by the reduction of cytochrome c in the presence of NADH at 550 nm [1]. Protein determination Protein content was determined by the Bradford test [20], using bovine serum albumin as standard. PHI ... explain the regulatory effect. In fact, on addition of PHI, the fluorescence of PHR-bound flavin increases. This finding points to a PHI-induced conformational change...

Ngày tải lên: 21/02/2014, 00:20

... metabolism in the cold. Mathemati- cal modelling and simulation of cold- induced metabolic reprogramming indicated major differences in the rates of interconversion between the pools of hexoses and sucrose, ... assessment of the trajec- tory of interconversion rates as a function of time of cold exposure, (b) comparison of the magnitudes of the vari...

Ngày tải lên: 14/02/2014, 22:20

... cloning the PCR fragments into the His-tag vector, pET-15b. The recombinant proteins were generated in the E. coli strain Origami (DE3), and the N-terminal His-tagged proteins were purified using ... opening and cell swelling, an event that is probably reduced in extent by CsA. CsA specifically inhibits PTP opening by binding to cyclophilin D in the matrix and on th...

Ngày tải lên: 16/02/2014, 09:20

... upon the distance between and relative ori- entation of two fluorophores, being independent of the strength of protein–protein interactions. In the case of very weak interactions as described in ... constituting hybrid tetramers. (A) Structure of fluorescent chimeric GAPDH proteins and tetrameric structure of GAPDH. The structure of chimeric proteins is s...

Ngày tải lên: 16/02/2014, 09:20