Tài liệu Báo cáo khóa học: Heterogeneity of homologously expressed Hypocrea jecorina (Trichoderma reesei ) Cel7B catalytic module pptx

... Sweden The catalytic module of Hypocrea jecorina (previously Trichoderma reesei) Cel7B was homologously expressed by transformation of strain QM9414. Post-translational modi- fications in purified Cel7B ... pI 5.8 5). Lane 2, purified Cel7B cat ; lane 3: purified Cel7B cat after Endo H treatment. Ó FEBS 2004 Heterogeneity of H. jecorina (T. reesei) Cel7B (Eur....

Ngày tải lên: 19/02/2014, 12:20

... (dimethylsulfoxide-d 6 ) d: 13.25 (br, 1H, NH), 8.44 (s, 1H, 2-H), 8.13 (d, J ¼ 2 Hz, 1H, 4-H), 7.57 (d, J ¼ 2 Hz, 1H, 6-H), 4.04 (s, 3H, OCH 3 ). MS (EI): 193 (M + ). 1j: (202 mg, 98 %), m.p. 146–148 ... nitrobenzimidazole 1c (A) and nitrothiadiazole 2b (B), with respect to methyltetrahydrofolate (MTHF), at inhibitor concentrations of 1000 l M ( ), 500 lM (m), 100 lM (d) and...

Ngày tải lên: 19/02/2014, 05:20

... the pres- ence of (A) 100 l M free Ca 2+ (N = 7 8), (B) 10 nM free Ca 2+ (N = 5 4), (C) 10 nM free Ca 2+ and 10 lM TG, after 30 min incuba- tion (N = 8 2). The lines are Gaussian fits of the height ... rate constant of the nucleotide-induced conforma- tional change: F(t)=C 1 k 1 exp () k 1 t), where F(t)is the number of elevated conformation with a lifetime t, C 1 is the numbe...

Ngày tải lên: 14/02/2014, 14:20

... treatment groups were studied: (a) vehicle- treated sham (sham); (b) GdCl 3 -treated sham (GdCl 3 ); (c) vehicle-treated CLP (CLP); and (d) GdCl 3 -treated CLP (GdCl 3 + CLP). CLP Polymicrobial sepsis ... Woburn, MA, USA); iNOS (Transduction Laboratories, San Jose, CA, USA); TLR4 and TLR2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA); and HMGB1 (Abcam, Cambridge, MA, USA). Binding of a...

Ngày tải lên: 14/02/2014, 18:20

... only after partial unfold- ing of the a-helix from the body of the b-sheet (Fig. 2C). Possible role of domain-swapping in the ‘off-pathway folding’ (OFF) model The OFF model for amyloid formation ... basis of the main features of the models, we have classified them into three groups (Table 1): (a) templat- ing and nucleation; (b) linear, colloid-like assembly of spherical oligome...

Ngày tải lên: 14/02/2014, 18:20

... incubation. a-Synuclein incubated (A) alone (k = 245 nm), (B) in the presence of 100 l M MPTP (k = 245 nm), (C) in the presence of 200 l M MPTP (k = 245 nm), (D) in the presence of 100 l M MPTP, dissolved ... expected diameter of the protein (< 20 nm) (Fig. 6A). In the presence of 100 lm (Fig. 6B) and 200 lm (Fig. 6C) MPTP and 100 lm (Fig. 6D) and 200 lm (Fig. 6E) MPP + , the siz...

Ngày tải lên: 14/02/2014, 19:20

... transition pore (PTP) [1,2], is of a sufficient size (cut-off of $ 1.5 kDa) to allow the pas- sage of solutes and water, which may also result in rupture of the outer membrane. The identity of the proteins ... F 0 F 1 -ATP synthase activity ( %) Increase in ADP–ATP exchange rate, mediated by the ANT ( %) +30 +1.38 +1.94 +3.65 +7.7 +1.70 a DWm (mV) )1 57 )1 54 )1 47 )...

Ngày tải lên: 14/02/2014, 19:20

... highest rate (22 of 2 3) by wild-type H. pylori, fol- lowed by the vacA mutant (19 of 2 8). No ulcers were found in gerbils infected with the cagE mutant (0 of 2 7) or in controls (0 of 2 7). Intestinal ... (1 1). In this manner, the bacteria could access integrins and inject CagA (1 2). Injected CagA can then induce the massive disruption of cell junctions (1 3) an...

Ngày tải lên: 14/02/2014, 19:20

... to vehicle (Ctrl), 600 lM of stearate (SA), 600 l M oleate (OA) or a combination of the two (SA ⁄ OA) in the absence or presence of 0.5 lM TrC, a specific inhibitor of ACS. (A) Cells were stained ... proce- dures. Results are expressed as percentages of the respective con- trols (Ctrl) (*P < 0.0 5). (C, D) The conditions were exactly as in (A) and (B), respectively, except tha...

Ngày tải lên: 14/02/2014, 22:20

... ND, not determined. Acceptor GS GSGB k cat (s )1 ) K m Acc (mM) k cat ⁄ K m Acc (s )1 ÆmM )1 ) k cat (s )1 ) K m Acc (mM) k cat ⁄ K m Acc (s )1 ÆmM )1 ) Methyl a- D-glucopyranoside 39 ± 8 a 70 ± ... 2– 6) were pro- duced (Fig. 5B); (d) leucrose production increased with a delay time ( 30 min); and (e) the final yield of saccharide was similar to that...

Ngày tải lên: 14/02/2014, 22:20