Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx

... purify CDK4 Y17 kinases from HeLa cell extracts, and found that the cellular phosphorylation of CDK4 on Y17 is mediated by Src family kinases. Results Detection of CDK4 Y17 phosphorylation and kinase ... CDK4 ⁄ cyclin D kinase activity is controlled is phosphorylation at tyrosine 17 (Y17) of CDK4. This site corresponds to tyrosine 15 (Y15) of CDK1...

Ngày tải lên: 18/02/2014, 18:20

... oligonucleosome self-association: dependence on divalent cations and core histone tail domains. Biochemistry 35, 40 09 40 15. 42 Guo CY, Wang Y, Brautigan DL & Larner JM (1999) Histone H1 dephosphorylation ... Phosphory- lation of linker histones by DNA -dependent protein kinase is required for DNA ligase IV -dependent ligation in the presence of histone H1. Proc Natl Acad...

Ngày tải lên: 19/02/2014, 06:20

... effect of bis-ANS upon PKA phosphorylation of HSL. The interaction of HSL with bis-ANS was found to have a K d of 1 lm in binding assays. Upon PKA phosphory- lation, the interactions of HSL with ... indicating that the bis-ANS interac- tion with HSL is altered upon phosphorylation of HSL by PKA (Fig. 2). HSL interaction with bis-ANS To evaluate the binding of bis-ANS to...

Ngày tải lên: 18/02/2014, 11:20

... the activation energy of 28 ± 3 kcalÆmol )1 observed for the P79S mutant tetramer formation is consistent with the contribution of one proline isomerization event, most likely the conversion of Pro 74 from ... most of the possible conditions. Is it likely that there is only one generic mechanism of amyloid fibril formation? Is there a common mechanism of pro- tein folding?...

Ngày tải lên: 14/02/2014, 18:20

... years ago [4] . On the basis of this long period of co-evolution, there are some indica- tions that colonization by H. pylori could have been beneficial for their human carriers and this probably provided ... computational predic- tions suggest that proteins encoded by HP0522 and HP0535 may be novel T4SS effectors [4] . Whether HP0522 and HP0535 are indeed translocated by the T4SS...

Ngày tải lên: 14/02/2014, 19:20

... demonstrated that KLF4 is required for the expression of VSMC differentiation marker genes induced by all-trans retinoic acid [4] ; KLF4 could induce inhibition of proliferation of VSMC, which is mechanistically ... Further- more, this transactivation was almost abolished upon further point mutations of the corresponding KLF4 binding site. The specificity of transcriptional act...

Ngày tải lên: 18/02/2014, 04:20

... Remarkable stabilization of a psychrotrophic RNase HI by combi- nation of thermostabilizing mutations identified by sup- pressor mutation method. Biochemistry 47 , 8 040 –8 047 . 14 Kimura S, Kanaya S ... denaturation of these proteins was reversible in this condition. The urea concentration of the midpoint of the urea-induced denaturation curve (C m ), the mea- surement of...

Ngày tải lên: 18/02/2014, 13:20

... closed P-loop conformation is stable over a broad ionic strength range. Structure of PhyAsr upon oxidation of the catalytic cysteine A systematic comparison of the open P-loop confor- mation in PhyAsr ... angles in Ala2 54 (/ ⁄ w = )88.7 ⁄ )19.7 to /⁄ w = ) 146 .5 ⁄ 136 .4) and the w torsion angle of Gly255 (18.9 to )157.9) upon oxida- tion. This large rotation of the peptide bon...

Ngày tải lên: 18/02/2014, 18:20

... Effects of methylmercury in vitro on methionine synthase activity in various rat tissues. Bull Environ Contam Toxicol 45 , 649 –6 54. 17 Kenyon SH, Nicolaou A & Gibbons WA (1998) The effect of ethanol ... inhibition; methionine synthase; molecular modelling Correspondence A. Nicolaou, School of Pharmacy, University of Bradford, Richmond Road, Bradford BD7 1DP, UK Fax: +44 12...

Ngày tải lên: 19/02/2014, 05:20

... interaction by site-directed mutagenesis. Residual endoxylanase inhibition activities of the TAXI His3 74 mutants were determined. The specific contribution of TAXI-I His3 74 in endoxylanase interaction ... Discussion Recombinant expression, purification and characterization of TAXI [H374A/K/Q] mutants The role of TAXI-I His3 74 in endoxylanase inhibition was studied by site-dire...

Ngày tải lên: 19/02/2014, 07:20