Tài liệu Báo cáo khoa học: Modulation of F0F1-ATP synthase activity by cyclophilin D regulates matrix adenine nucleotide levels pptx

... the difference of DWm (termed Delta phi) before and after the addition of ADP (2 mm) to WT and CYPD KO mitochondria, and cal- culated on the basis of steady-state titration data by catr and olgm. ... of inhibition by cyclophilin D was evident in the form of slightly increased respiration rates during arsenolysis. However, the modulation of F 0 F 1 -ATP synthase by c...

Ngày tải lên: 14/02/2014, 19:20

... intensity indeed denoted the formation of higher molecular mass aggregates, SDS ⁄ PAGE and immuno- blotting were carried out according to the procedure described in Materials and methods. a-Synuclein ... containing MPTP (A and C) and MPP + (B and D) were analysed by SDS ⁄ PAGE (A and B) on 5–15% crosslinked polyacrylamide gel and western blotting (C and D) . (A, B) Lane M, prestained mo...

Ngày tải lên: 14/02/2014, 19:20

... exopeptidase activity of cathepsin B in the presence of 2A2 mAb. Furthermore, 2A2 mAb also inhibited cathepsin B endopeptidase activity, as determined by the degradation of DQ-col- lagen IV and BODIPY ... (A), degradation products are visible intracellularly and pericel- lularly (white arrow). Addition of the 2A2 mAb to the assay medium reduced the degradation of DQ-collagen IV...

Ngày tải lên: 18/02/2014, 11:20

... analysis of cells lacking STE20 and CLA4. Data are mean values with standard deviation from at least two independent experiments. lg of sterol per mg of protein wild-type ste2 0D cla 4D swe 1D cla 4D swe 1D Ergosterol ... morphology of the strains used in this study. (A) Morphology of deletion strains. The indicated strains were grown in YPD to stationary phase. The cells were...

Ngày tải lên: 18/02/2014, 13:20

... of FNR for oxidized Fd decreases by more than 10-fold on addition of NADP + [60]. Considering that molecular modeling indicates that neither NADP + nor the residues C266, G267 and L268 are adjacent ... amino acid groups introduced by mutations was calculated following the standard radii and volumes calculated by Tsai et al. [32], assuming a reduced state of the cysteine. Amino aci...

Ngày tải lên: 18/02/2014, 17:20

... and 14 show the PCR products obtained using the primer pair T7 and ADC2. Lane 15, 1 kb DNA ladder standard. (B) DNA obtained from arginine decarb- oxylase (ADC)-transformed parasites harvested ... plasmid had almost disappeared (Fig. 3A, lanes 7 and 8). The faint PCR band of  3500 bp detected in Fig. 3A (lane 8), in addition to the expected 2030 bp PCR product, might be gener- ated by a...

Ngày tải lên: 19/02/2014, 07:20

... action of EPA and DHA (Eur. J. Biochem. 271) 4465 Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the e arliest effects of mitogenic ... of n-3 PUFAs (EPA or DHA) d id not stimulate the basal cyclin D1 gene promoter activity. EPA and DHA also reduced the increased cyclin D1 activity in response t...

Ngày tải lên: 19/02/2014, 16:20

... emulsified substrate was added and mixed. Reactions were typically incubated for a period of 30 min at 37 °C before the reaction was quenched by the addition of 3.25 mL of methanol ⁄ chloroform ... measurements. Spectra of samples containing phosphorylated and dialysed HSL mixed with bis-ANS were recorded, and spectra of dialysed phosphorylation mixes (including PKA, which pro- vid...

Ngày tải lên: 18/02/2014, 11:20

... started by the addition of 10 l L of NADPH (0.75 mm final concentration), and continued for 30 min at 37 °C. To determinate the Ca 2+ -independent activity of NOS (i-mtNOS), 10 mm EDTA was added to ... serial dilutions (50, 5, 0.5 and 0.05 ng), and were normalized by b-actin expression. Nitrite determination Mitochondrial fractions were thawed and suspended in ice- cold distilled w...

Ngày tải lên: 19/02/2014, 00:20

... second antibody against the extracellular domain of E-cadherin, which did not disrupt cell–cell adhesion, did not induce Erk activation. Second, inhibition of Src blocked the disruption of cell–cell ... up-regulated upon disruption of E-cadherin-dependent cell–cell adhesion. We explored the underlying molecular mechanisms and found that blockage of E-cadherin by Decma elicits a si...

Ngày tải lên: 19/02/2014, 05:20